Fig. 5

HPDAlR showed much more potent pro-regenerative activity than RL-QN15 against acute full-thickness injured skin wounds in mice. A Typical skin wound images of mouse topically treated with vehicle (PBS), HPDA, RL-QN15, HPDA (0.1 mg/mL)lR(1 nM) or HPDA (0.2 mg/mL)lR(1 nM) on postoperative days 0, 2, 4, 6, and 8. B Quantitative curves of pro-healing activities of Vehicle (PBS), HPDA, RL-QN15, HPDA (0.1 mg/mL) lR (1 nM), HPDA (0.2 mg/mL)lR(1 nM) groups. Error bars represented SD. ^##P < 0.01, ^###P < 0.001and ^####P < 0.0001 indicated statistically significant differences compared to vehicle. ^**P < 0.01, ^***P < 0.001 and ^****P < 0.0001 indicated statistically significant differences compared to RL-QN15 (Student t-test). C Histopathological examination of excisional wounds stained with H&E. NE, Neoepidermis; GT, Granulation tissue; ES, Eschar; M, Muscle; Scale bar: 200 μm. D and F. Quantitative analysis of neo-epidermal thickness on postoperative days 4 and 8. E and G Quantitative analysis of granulation thickness on postoperative days 4 and 8, respectively. Data were Mean ± SD (n = 9), error bars represent SD. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 indicated statistical differences between two groups (Student t-test). H and I Effects of local HPDAlR treatment on TGF-β1 content in skin wounds on post-operation day 4 and day 8. Data were Mean ± SD from three independent experiments performed in triplicate. *P < 0.05 indicated statistical differences between two groups (Student t-test)