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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Understanding of catalytic ROS generation from defect-rich graphene quantum-dots for therapeutic effects in tumor microenvironment

Fig. 3

Catalytic performance of GQDs. a Michaelis–Menten kinetic profile. b Catalytic performance comparison via relative reaction velocity from TMB assay (in Hac/NaAc buffer) for GQDs (100 µg/ml) with different treatments (untreated, GQDs-300, GQDs-500) under TME (100 µM H2O2), insert: photo of TMB colorimetric assay at 10 min post-treatment of GQDs, GQDs-300, GQDs-500. c Fluorescence spectra of TA, GQDs, TA (TME) and GQDs + TA (TME) in PBS. The concentration of TA, TME (H2O2) and GQDs in PBS were 1 mM, 100 µM and 100 µg/ml, respectively. Hydroxyl radical identification via EPR using DMPO (1 mM) as the spin trap of: d GQDs (50 µg/ml) with and without H2O2 (1 mM), and e GQDs + H2O2 with and without adding MCF-7 cells (1 × 106/ml). f Relative hydroxyl radical concentrations calculated from the signals in using Xenon quantify EPR software

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