Fig. 8

In vivo fluorescence imaging of SW620 tumor-bearing nude mice after administration with DiD, DiD-BBA/CM-β-CD, and DiD-BBA/FA-PEG-CM-β-CD. A Fluorescence distribution observed using an in vivo imaging system at 3, 8, and 12 h post-administration. B Ex vivo fluorescence imaging of the heart, liver, spleen, lung, kidney, and tumor tissue of SW620 tumor-bearing mice at 12 h post-administration. Semi-quantitative analysis of C the in vivo fluorescence intensity at three-time points and D ex vivo fluorescence intensity of tissues using ImageJ. Data were expressed as mean ± standard deviation (n = 5). Two-way ANOVA (**P < 0.01, ***P < 0.001, ****P < 0.0001) was used to assess the statistical differences between the treatment groups