Fig. 2

Preparation and characterization of DMSN-PEI@125a nanocomposites. A Schematic illustration of DMSN-PEI@125a preparation and redox-responsive release in cells. B TEM image of DMSN-PEI@125a. C Particle size distribution of DMSN-PEI@125a measured by DLS. D Zeta potential of DMSN-PEI@125a (mass ratio of DMSN-PEI to miR-125a is 100:1). E UV absorption spectrum of DMSN-PEI and DMSN-PEI@125a. F miR-125a loading capacity and G RNase A protection assay detected by agarose gel electrophoresis. Cellular uptake of DMSN-PEI@125a and free miR-125a in vitro and in vivo. H Fluorescence confocal microscopy images of free miR-125a and DMSN-PEI@125a uptake by RAW264.7 cells and TC-1 cells in at different time points. Bar = 25 μm. I Tumor bearing C57BL/6J mice were i.t. injected with DMSN-PEI@125a. The uptake of miR-125a by TAMs (CD11b positive) or tumor cells (CD11b negative) in tumors were detected at 48 h using flow cytometry. J Quantitative percentage of miR-125a-Cy5 positive cells in CD11b positive or negative cells