Fig. 4
From: Multifunctional silica nanocomposites prime tumoricidal immunity for efficient cancer immunotherapy
DMSN-PEI@125a induce TC-1 cell apoptosis and immunogenic death in vitro. A, B Cytotoxic effect of miR-125a and DMSN-PEI@125a on TC-1 cells at different concentrations for A 24 h and B 48 h. TC-1 cells were treated with DMSN-PEI, DMSN-PEI@NC, DMSN-PEI@125a, and miR-125a for 48 h. (concentration of miR-125a or NC is was 250 × 10−9 M, mass ratio of DMSN-PEI to miR-125a is 25:1). C Representative flow cytometry images and D percentage analysis of TC-1 apoptosis. E Expression levels of apoptosis-related proteins (STAT-3, caspase-3, caspase-7, caspase-9) were detected by western blotting. F mRNA expression level of calreticulin (CRT) in TC-1 cells was detected using qPCR