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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Hemin enhances the cardioprotective effects of mesenchymal stem cell-derived exosomes against infarction via amelioration of cardiomyocyte senescence

Fig. 6

Exosomal miR-183-5p in Hemin-MSC-EXO inhibited mitochondrial fission via regulation of HMGB1. A Bioinformatics analysis predicted the binding sites between miR-183-5p and HMGB1, confirmed by dual-Luciferase reporter assay. B RT-PCR analysis of the level of miR-183-5p in control, miR-183-5p mimic and miR-183-5p inhibitor-treated NMCMs. C RT-PCR analysis of the level of HMGB1 in control, miR-183-5p mimic and miR-183-5p inhibitor-treated NMCMs. D Western blotting analysis of HMGB1 level in control, miR-183-5p mimic and miR-183-5p inhibitor-treated NMCMs. E Western blotting analysis of the level of HMGB1, p-Drp1, Drp1, p-ERK, ERK in control, SD/H, SD/H + Hemin-MSC-EXO, SD/H + miR-183-5pKD-Hemin-MSC-EXO and SD/H + miR-183-5pKD-Hemin-MSC-EXO + U0126 treated NMCMs. n = 3 biological replicates for each group. Data are expressed as mean ± SD. **p < 0.01, ***p < 0.001, ns = not significant

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