Fig. 3

The corneal penetration behaviors of nanomedicines. A Representative in vivo two-photon microscopy 2D images of corneal cross-sections at 1 h after administration of NM and PNM in C57Bl/6 mice; B Representative in vivo two-photon microscopy 3D images of corneal after administration PNM in C57Bl/6 mice; C Representative micrographs of corneal sections obtained from each group stained with DAPI (blue fluorescence) and anti-β-catenin (green) at 1 h and D 12 h after topical application. DAPI staining, blue. Merge, colocalization image of β-catenin and DAPI; E Confocal laser scanning microscopy images of HCECs cell layers immunofluorescent stained with DAPI (blue fluorescence) and ZO-1 (green fluorescence) after incubation with CNM and CPNM for 1 h and F the statistical analysis results. DAPI staining, blue. Merge, colocalization image of ZO-1 and DAPI. Bar = 50 μm