Fig. 9From: Celastrol-based nanomedicine promotes corneal allograft survivalCPNM attenuated cytokines secretion in M1 macrophages. A Detection of cytokine expression profiles in supernatants from LPS-inducing peritoneal Mϕ. Supernatants from TLR4−/− Mϕ and wild-type Mϕ pretreated with CPNM, ST2825 and BAY11 for 24 h and normal medium (control) were assessed using a mouse cytokine antibody array. The downregulation of IL-6, IL12p40/p70, IFN-γ, MCP-1, RANTES and TNFR I was shown in the supernatants from LPS-inducing wild-type Mϕ after CPNM pretreatment. There was no significant difference in the expression of IFN-γ and TNFR I in LPS-inducing TLR4−/− Mϕ with CPNM. B Statistical analysis of relative protein levels of cytokines. Mean densities of protein were calculated by normalizing to controls. The data indicates the mean ± SD (n = 3), *P < 0.05 and **P < 0.01Back to article page