Fig. 4

Tumor penetration and biodistribution of nanocarriers in vivo. A tumor sections of tumor-bearing nude mice 24 h after injection with the four types of nanocarriers. Blue: DAPI; green: CD34; red: nanocarriers. B In vivo real-time fluorescence images of tumor-bearing nude mice before and after injection with four types of nanocarriers. The black circles show the tumor areas. C Ex vivo organ fluorescence images of tumors and various organs 24 h postinjection. D Fluorescence intensities of various organs 24 h postinjection. *P < 0.05, **P < 0.01 was analyzed by one way analysis of variance (ANOVA). (I: FA-MSNR/LMDI@MP/MPCM; II: FA-MSNR/LMDI; III: FA-MSNR/LMDI@MPCM; IV: FA-MSN/LMDI)