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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Tumor microenvironment-activated cancer cell membrane-liposome hybrid nanoparticle-mediated synergistic metabolic therapy and chemotherapy for non-small cell lung cancer

Fig. 2

Intracellular NP internalization. a Flow cytometry analysis of the amount of Nile red internalized by A549 cells after 4 h treatment. b Quantitative analysis of mean fluorescence intensity of Nile red (n = 3). c Flow cytometry analysis of the amount of FAM-siPGAM1 internalized by A549 cells after 4 h treatment. d Quantitative analysis of mean fluorescence intensity FAM (n = 3). e Confocal microscopy images of A549 cells after a 4 h treatment with Nile Red, FAM-siPGAM1, LC-Nile Red, LC-FAM-siPGAM1, CLip-PC@LC-Nile red (with and without 50 nM MMP-9), CLip-PC@LC-FAM-siPGAM1 NPs (with and without 50 nM MMP-9), or CLip-PC@CO-LC NPs (with and without 50 nM MMP-9) for 4 h. Green, FAM-labeled siPGAM1. Red, Nile red. Blue, nuclei dyed with DAPI. Scale bars, 10 μm. f Confocal images of A549 cells treated with CLip-PC@CO-LC NPs (MMP-9) for different durations (2 h, 4 h). The green fluorescence of FAM-siPGAM1 is displayed. DAPI (blue) was used to visualize nuclei, and LysoTracker red (red) was used to label the endo/lysosome. Scale bars, 10 μm

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