Fig. 4
HIFU ablation induced PLD@NE migration to the tumor site. a Schematic illustration of HIFU ablation in tumor-bearing mice. b Ablation efficiency verified by CEUS in real time. Before HIFU, the perfusion of the tumor was enhanced overall, suggesting that it was a solid tumor with abundant blood flow. After HIFU, a perfusion defect area appeared in the tumor, indicating ablation damage. c, d Expression levels of CXCL1/KC and IL-10 in tumors (c) and serum (d) of mice over 48 h after HIFU ablation. n = 3. e In vivo fluorescence images of tumor-bearing mice after intravenous administration of DiR-PLD or PLD@DiR-NEs over time. Mice were treated with (−) or without (+) HIFU ablation. PLD@DiR-NEs were injected at a dose of 3 × 106 cells per mouse. The tumor site is indicated by the dashed circle. DiR-PLD, DiR labeled PLD; DiR-NEs, DiR labeled neutrophils