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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Gene augmented nuclear-targeting sonodynamic therapy via Nrf2 pathway-based redox balance adjustment boosts peptide-based anti-PD-L1 therapy on colorectal cancer

Fig. 4

in vitro SDT efficiencies of TIR@siRNA in CT26 cells. Fluorescence images (A), flow cytometry curves (B) and quantitative analysis (C) of the generations of intracellular ROS after various treatments. Confocal images (D) and western blot analysis (E) of expressions of Nrf2 of various treatments. Confocal images (F) and flow cytometry analysis (G) and quantitative analysis in Q3 quadrant (H) of the membrane potential changes of mitochondrial stained with JC-1 after various treatments. I Confocal images of the cells after 24-hour treatments of free IR780, TIR and TIR@siRNA with or without US irradiation. Mitochondria and Cyt c stained respectively with MitoTracker green (M7514) and anti-Cyt c antibody emitted green and red fluorescence. J Relative released Cyt c from mitochondrial to cytoplasm (the control was set as 1). *P < 0.05 and **P < 0.01 for the comparisons between two treatment groups

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