Fig. 2

Targeted imaging and subcellular localization of tumor cells by TB/PTX@RTK micelles in vitro. A, B CLSM images and relative semi-quantitative analysis of the fluorescence intensity of L-O2, HK-2, Hep G2 and Hep 1–6 cells after incubated with the micelles (5 μg/mL) for 4 h. Red fluorescence is emitted by the micelles. Green fluorescence and blue fluorescence label the cytoskeleton and the nuclei, respectively. Scale bar: 20 μm. C, D Confocal images and semi-quantitative analysis of Hep G2 after incubation with TB/PTX@TK or TB/PTX@RTK micelles (5 μg/mL) for 4 h without or with pretreatment with cilengitide. Scale bars: 50 μm. E, F Confocal images and co-localization analysis of TB/PTX@RTK micelles with lysosomes. Scale bar: 10 μm