Fig. 3

Fluorescence imaging, biodistribution analysis and pharmacokinetics of TB/PTX@RTK micelles in vivo. A Fluorescent images of the Hep 1–6 tumor-bearing Balb/c nude mice at different time points after injection of TB/PTX@RTK micelles (5 mg/kg). The white dotted ellipse indicates tumor sites. B Ex vivo fluorescence imaging of tumor tissues and major organs from tumor-bearing mice 12 h after intravenous injection of micelles. C Biodistribution of TB/PTX@RTK micelles in tumor-bearing mice 12 h after injection of TB/PTX@RTK micelles (5 mg/kg) without or with pretreatment with cilengitide. The white dotted ellipse indicates tumor sites. D Quantitative analysis of organ distribution of TB/PTX@RTK micelles in tumor-bearing mice with or without pretreatment with cilengitide. Data represent mean ± SD (n = 3). **p < 0.01. E Pharmacokinetics of free TB and TB/PTX@RTK micelles in the blood. Data represent mean ± SD (n = 3)