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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Intracellular signaling pathway in dendritic cells and antigen transport pathway in vivo mediated by an OVA@DDAB/PLGA nano-vaccine

Fig. 3

Cytokine mRNA expression and activation of relevant signal pathway profiles elicited by different vaccine adjuvants for BMDCs. a, b, c The cytokine (IL-1β, IFN-γ and TNF-α) mRNA expression from BMDCs was analyzed by RT-qPCR. d The changes of p38 MAPK, p-AKT and p-ERK phosphorylation after being stimulated with different formulation DCs for 6 h and 12 h. e Change of the p38 MAPK, p-AKT and p-ERK phosphorylation level by stimulated DCs with different concentration of DDAB-PLGA Nv. f DDAB-PLGA Nv increased the binding of MKK3 to its substrate p38α. g The ability of AP2α to recruit TNF-α and IL-1β promoters in the P38 pathway. h Illustration of how OVA@DDAB/PLGA Nv promoted DCs activation and maturation via the p38 MAPK signaling pathway. i After treatment with inhibitors of p38 (SB203580), the change of OVA@DDAB/PLGA Nv induced cytokine secretion from BMDCs. j After treatment with inhibitors of p38 (SB203580), the change of OVA@DDAB/PLGA Nv induced cytokine mRNA expression from BMDCs. Data are expressed as Mean ± STD (n = 6). (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)

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