Fig. 5
Dynamic antigen biodistribution of different vaccine formulations in mice. a Experimental design to evaluate the dynamic antigen biodistribution. b–f 89Zr-OVA biodistribution in injection site (b), spleen (c) Popliteal LNs (d), inguinal LNs (e), and cervical LNs (f). The primary organs and secondary lymphoid tissues of mice were isolated at 0.5, 3, 6, 12, 24, 48, and 72 h, and then radioactivity was quantified to calculate %ID/g value (% ID/g: antigen uptake rate per gram of tissue at different times). g immunohistochemical methods quantified the antigen in draining LNs (the brown areas represent the antigen OVA), and stained sections were measured by the automatic multispectral imaging system (PerkinElmer Vectra II), bar = 200 μm. Data are expressed as Mean ± STD (n = 6)