Fig. 1
From: RETRACTED ARTICLE: A stepwise-targeting strategy for the treatment of cerebral ischemic stroke
Melatonin derivative TPP-MLT and its antioxidant activity. A Synthetic route of TPP-MLT (compound 3). B 1H NMR spectrum of TPP-MLT (500 MHz, Chloroform-d). C Mass spectrum of TPP-MLT. D The MLT content in mitochondria of PC-12 cells treated with MLT and TPP-MLT (n = 6, **P < 0.01, *P < 0.05). E Expression levels of ROS in PC-12 cells treated with MLT and TPP-MLT. DCFH-DA fluorescent probes as ROS indicator. Scale bar, 50 μm. F The changes of mitochondrial membrane potential in PC-12 cells treated with MLT and TPP-MLT. Scale bar, 50 μm. G Cell viability of PC-12 cells treated with MLT and TPP-MLT (n = 6, *P < 0.05). H PC-12 cells were stained with calcein AM (green) and EthD-1 (red) to visualize live and dead cells. Scale bar, 200 μm