Fig. 5
From: RETRACTED ARTICLE: A stepwise-targeting strategy for the treatment of cerebral ischemic stroke
In vivo efficacy of TGN/SHp/TPP-MLT micelles. A Expression levels of ROS in PC-12 cells treated with saline, TPP-MLT and TGN/SHp/TPP-MLT micelles. DCFH-DA fluorescent probes as ROS indicator. Scale bar, 50 μm. B The changes of mitochondrial membrane potential in PC-12 cells treated with saline, TPP-MLT and TGN/SHp/TPP-MLT micelles. Scale bar, 50 μm. C Cell viability of H2O2-stimulated PC-12 cells after treated with TGN/SHp/TPP-MLT micelles, free TPP-MLT as control (n = 6). D PC-12 cells were stained with calcein AM (green) and EthD-1 (red) to visualize live and dead cells. Scale bar, 200 μm. E Semi-quantitative fluorescent analysis of live and dead cells. F Flow cytometry-based apoptosis assay by the Annexin V-FITC Apoptosis Kit. PI, propidium iodide. G Western blot analysis of Cytochrome C in Mitochondria and Cytosol, Cleaved Caspase-3 and Cleaved Caspase-9 in H2O2-stimulated PC-12 cells treated with TGN/SHp/TPP-MLT micelles, free TPP-MLT as control. H–K Densitometric analysis of the western blot bands (n = 3, *P < 0.05)