Fig. 7

The role of Cur@EVs^−cl−NGF in the early stages of injury. A Flow cytometry analysis of the ratio of M1 microglia/macrophages to total microglia/macrophages. (Numbers indicated per cent F4/80 + , CD86 + cells). B Flow cytometry analysis of the ratio of M2 microglia/macrophages to total microglia/macrophages. (Numbers indicated per cent F4/80 + , CD206 + cells). C Fluorescence images of spinal cord tissue, in which the green channel represented neurons (NurN) and the red channel represented apoptosis marker protein (c-caspase3). D ELISA-based detection of proinflammatory factors (TNF-α, IL-1β, IL-6) and anti-inflammatory factors (TGF-β) in the injured spinal cord tissue. E, F Fluorescence quantitative statistical analysis of NurN and c-caspase3 in figure C. * at the top of each group's statistical graph indicates the difference analysis with the NS group. Data presented the mean ± SD (n = 6 per group) (*P < 0.05, **P < 0.01, ***P < 0.001 and ns: not significant)