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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Extracellular vesicles derived from hypoxia-preconditioned olfactory mucosa mesenchymal stem cells enhance angiogenesis via miR-612

Fig. 5

OM-MSC-EVs transferred miR-612 modulates HBMECs specification through TP53. A qPCR analysis of the expression of TP53, HIF-1α and VEGF in HBMECs treated with H-EVs and N-EVs, (n = 6), **P < 0.01, ***P < 0.001. B Western blot analysis of the expression of TP53, HIF-1α and VEGF in HBMECs treated with H-EVs and N-EVs. C Quantitative data of western blot analysis, (n = 3),*P < 0.05, ***P < 0.001. D The bioinformatic tool TargetScan to identify putative TP53 was one of predicted target of miR-612.miR-612 mimic significantly decreased the relative luciferase activity of the wild-type vectors, while the luciferase activity of mutant vectors was not altered by miR-612, (n = 3), ***P < 0.001, ns: no significance. E qPCR analysis of the expression of miR-612, TP53, HIF-1α and VEGF in HBMECs treated with each treatment group, (n = 6), ***P < 0.001. F Western blot analysis of the expression of TP53, HIF-1α and VEGF in HBMECs treated with NC mimics and miR-612 mimics,and quantitative data of western blot analysis, (n = 3), ***P < 0.001. G Western blot analysis of the expression of TP53, HIF-1α and VEGF in HBMECs treated with NC inhibitor and miR-612 mimics inhibitor,and quantitative data of western blot analysis, (n = 3), ***P < 0.001

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