Fig. 8

H-EVs promoting endothelial cell angiogenesis in vivo. A Schematic representation In vivo experimental design. B Macroscopic view of the Matrigel plug (Matrigel only group). The H-EVs treatment group can significantly promote the formation of blood vessels in the Matrigel plugcompared to the N-EVs treatment group, (n = 3), scale bar: 5 mm (for macroscopic images), scale bar: 1 mm (for box images), red arrows indicate blood vessels. C Macroscopic view of the Matrigel plug (Matrigel + HBMECs group). H-EVs and N-EVs treatment can promote blood vessel formation in the matrigel, (n = 3), scale bar: 5 mm (for macroscopic images), scale bar: 1 mm (for box images), red arrows indicate blood vessels. D The formation of branching capillaries in Matrigel (Matrigel only group), expressing CD31 (green), (n = 3), scale bar: 100 μm (for immunohistochemical images), scale bar: 50 μm (for box images), yellow arrows indicate blood vessels. E The formation of branching capillaries in Matrigel (Matrigel + HBMECs group), expressing CD31 (green), (n = 3), scale bar: 100 μm(for immunohistochemical images), scale bar: 50 μm (for box images), yellow arrows indicate blood vessels. F The meshse of a plug staining positively with anti-CD31was quantitated using image J (Matrigel only group and Matrigel + HBMECs group), (n = 6), **P < 0.01, ***P < 0.001. G The area of a plug staining positively with anti-CD31 was quantitated using image software (Matrigel only group and Matrigel + HBMECs group), (n = 6), **P < 0.01, ***P < 0.001. H Western blot analysis of the expression of TP53, HIF-1α, and VEGF in invading cells (Matrigel + HBMECs group) treated with H-EVs and N-EVs,and quantitative data of western blot analysis, (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001