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Fig. 9 | Journal of Nanobiotechnology

Fig. 9

From: Extracellular vesicles derived from hypoxia-preconditioned olfactory mucosa mesenchymal stem cells enhance angiogenesis via miR-612

Fig. 9

The miR-612 is abundant in H-EVs promoting endothelial cell angiogenesis in vivo. A Macroscopic view of the Matrigel plug. The Matrigel plug in the miR-612 agomir group showed much more vessels than other groups. H-EVs, and compared with N-EVs treatment, H-EVs can more significantly reverse the inhibitory effect of antagomir-612 on angiogenesis, (n = 3), scale bar: 5 mm (for macroscopic images), scale bar: 1 mm (for box images), red arrows indicate blood vessels. B Immunofluorescence staining observes the morphology and number of blood vessels in Matrigel plugs, (n = 3), scale bar: 100 μm (for immunohistochemical images), scale bar: 50 μm (for box images), yellow arrows indicate blood vessels. C The area of a plug staining positively with anti-CD31 was quantitated using image software, (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001. D–G Western blot analysis of the expression of TP53, HIF-1α, and VEGF in invading cells treated with each treatment group,and quantitative data of western blot analysis, (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001

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