Fig. 3
From: Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections
Sequence-specific bactericidal activity of CRISPR-Cas13a. A Schematic diagram of the transformation of CRISPR-Cas13a and CRISPR-Cas9 by targeting blaIMP-1 into blaIMP-1-expressing E. coli STBL3. B E. coli STBL3 expressing blaIMP-1 from a plasmid and chromosome was prepared and transformed with CRISPR-Cas13a or CRISPR-Cas9, both with a spacer targeting blaIMP-1 or no spacer (nontargeting). The resulting transformants were plated on an LB plate to test sequence-specific bacterial killing by CRISPR-Cas13a and CRISPR-Cas9. C. The number of bacteria on the plate obtained in experiment B was counted. Reprinted with permission from [65]