Fig. 3
Cell viability and DNA-repair-related genes’ expression in MNPs@SiO2(RITC) internalized cells. a MTS assay of MNPs@SiO2(RITC)-treated HEK293, NIH3T3, and RAW 264.7 cells. Cells were treated with 0.1 mg/mL, 0.3 mg/mL, and 1.0 mg/mL MNPs@SiO2(RITC) for 12 h, followed by cell viability assessment using an MTS assay. b Quantitative analysis of DNA repair-related genes using quantitative real-time PCR (qPCR). HEK293 cells were treated with 0.1 mg/mL and 1.0 mg/mL of MNPs@SiO2(RITC) for 12 h. qPCR was performed using specific primers for target genes CRACR2A, REST, DAZAP2, HERPUD1, and UBQLN4. Gene expression levels of the target genes were normalized relative to the corresponding means in non-treated controls. GAPDH was used as an internal control in qPCR. Here, *p < 0.05 and **p < 0.01 in one-way ANOVA compared to the control were considered significantly different