Fig. 4

ICD induction effects of PLNP^Cu in vitro. a, b Flow cytometric analysis of apoptosis rate of 4T1 cells after treatment with PBS, LOX, PLNP^Cu pH = 7.4 and PLNP^Cu pH = 6.5. c, d The flow cytometric analysis and quantitative results of relative fluorescence intensity of CRT on 4T1 cells. e Fluorescent microscopy image of CRT exposure. Scale bars, 100 μm. f Fluorescent microscopy image of HMGB1 outflow from the nucleus. Scale bars, 100 μm. g The ATP secretion from cancer cells after different treatments. Results were expressed as mean ± SD. The significant difference was calculated via one-way ANOVA analysis (b, d, g). (NS represented not significant, *p < 0.05, **p < 0.01, ***p < 0.001)