Fig. 8

Immune activation by PLNP^Cu in vivo. a The flow cytometric images of the CD4⁺ T cells and CD8⁺ T cells in spleen (gated on CD3⁺ T cells). b The flow cytometric images of the CD80⁺CD86⁺ DCs in LNs (gated on CD11⁺ DCs). c The flow cytometric images of the CD86⁺MHC II ⁺ DCs in LNs (gated on CD11⁺ DCs). d The flow cytometric quantification of the rate of CD8⁺/CD4⁺ T cells in spleen. e The flow cytometric quantification of the CD86⁺CD80⁺ DCs in LNs. f The CD86⁺MHC II⁺ DCs in LNs. Results were expressed as mean ± SD. The significant difference was calculated via one-way ANOVA analysis (d–f). (NS represented not significant, *p < 0.05, **p < 0.01, ***p < 0.001).