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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Spatiotemporal regulation of angiogenesis/osteogenesis emulating natural bone healing cascade for vascularized bone formation

Fig. 4

In vitro osteogenesis potential. A Representative ALP staining images on day 3. B Quantitative analysis of ALP activity of BMSCs. C Representative ARS images on day 14. D Quantitative analysis of calcium deposition of BMSCs. E, F Osteogenesis of BMSCs was measured by immunofluorescence assays for Runx2 expression on day 4, with the nucleus stained in blue and OCN stained in red. qRT-PCR analysis of osteogenesis-related gene expressions including G ALP, H Col-1, I Runx2. J Osterix and K OCN. Statistically significant differences are indicated with *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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