Fig. 2
Co-localization of Lyso-Tracker Green FM (blue) and Dil (red) for RF and CF over time in 4T1 tumor cells. B Dil fluorescence intensity of A determined using ImageJ software. C JC-1 (green) for JC-1 monomer and red for JC-1 aggregate fluorescence image under different treatment. D Nuclear condensation and DNA fragmentation were visualized using DAPI and γ-H2AX staining in cells treated as indicated, with representative pictures presented. E The density of γ-H2AX foci was determined based on analyses of 100 cells per treatment group (γ-H2AX foci/100 μm2, n = 3). F Colony formation assays were conducted using 4T1 cells treated with 2 or 6 Gy of radiation (n = 3). G The impact of FeS2 on the intracellular level of GSH was estimated using a GSH assay kit (n = 5). Significant differences among groups as calculated using the student’s t test. **P < 0.01, ***P < 0.005