Fig. 10

A Representative images of tumors collected from mice after various therapies (single DFO dose: 20 mg/kg). B–D Tumor volume, tumor weight, and mouse weight were measured during the therapeutic period. (The data are presented as the mean ± SD values; n = 5, *p < 0.05, **p < 0.01.) E–J CLSM and FC analyses of the intracellular ROS, LPO, and lipid-ROS levels by PDT after pretreatments of DFO and Fer-1. (The data are presented as the mean ± SD values; n = 3, *p < 0.05, **p < 0.01.) The scale bars represent 100 µm. K The decreased relative cell viability (%) of HOS cells of PDT via preincubation of DFO and Fer-1. (The data are presented as the mean ± SD values; n = 3, **p < 0.01). L After ferroptosis scavenging by DFO and Fer-1, the expression levels of cell apoptosis-related proteins induced by PDT were measured by western blot analysis. M–N The suppression of cell apoptosis percentages (stained with annexin V-FITC/PI) by the inhibition of ferroptosis as determined by FC. The data are presented as the mean ± SD values; n = 3, *p < 0.05.) PDT: laser + MH-PLGA-IR780 NPs