Skip to main content

Nanomaterials and nanotechnology for the delivery of agrochemicals: strategies towards sustainable agriculture


Nanomaterials (NMs) have received considerable attention in the field of agrochemicals due to their special properties, such as small particle size, surface structure, solubility and chemical composition. The application of NMs and nanotechnology in agrochemicals dramatically overcomes the defects of conventional agrochemicals, including low bioavailability, easy photolysis, and organic solvent pollution, etc. In this review, we describe advances in the application of NMs in chemical pesticides and fertilizers, which are the two earliest and most researched areas of NMs in agrochemicals. Besides, this article concerns with the new applications of NMs in other agrochemicals, such as bio-pesticides, nucleic acid pesticides, plant growth regulators (PGRs), and pheromone. We also discuss challenges and the industrialization trend of NMs in the field of agrochemicals. Constructing nano-agrochemical delivery system via NMs and nanotechnology facilitates the improvement of the stability and dispersion of active ingredients, promotes the precise delivery of agrochemicals, reduces residual pollution and decreases labor cost in different application scenarios, which is potential to maintain the sustainability of agricultural systems and improve food security by increasing the efficacy of agricultural inputs.

Graphical Abstract


Particles with nanometers sizes in at least one dimension are commonly known as nanomaterials (NMs) [1, 2]. Due to their small size, NMs are used in many fields, such as life sciences, aerospace, electronics, chemical production, and agriculture. Many inorganic nanoparticles are employed as NMs, for example, carbon nanotubes are usually used as carriers; Iron nanoparticles are widely used due to their magnetism; Silica nanoparticles have attracted research attention for their abundant pore structure and large specific surface area. Other studies have focused on copper, gold, or silver nanoparticles [3]. Polymers and liposomes are renewable, biodegradable, and environmentally friendly, usually used as organic carriers to encapsulate pesticides [4, 5]. These materials are also called nanostructured materials (NSMs) or engineered nanomaterials (ENMs), and most applications are concentrated on efficiency and productivity enhancement.

Roco et al. were the first to note that NMs can be used for drug delivery [6]. A National Planning Workshop of America proposed the use of NMs in agriculture, mainly for rapid detection of pesticide residues and food packaging [7]. Subsequent research on the application of NMs has involved various aspects of agrochemicals such as pesticide formulations [8, 9], pesticide residues analysis [10, 11], fertilizers [12, 13], plant growth regulators (PGRs) [14], and development of bio-pesticides [15, 16], including nucleic acid pesticides and pheromone. The application of NMs in agrochemicals is shown in Fig. 1. In 2019, IUPAC selected ten chemical technologies most likely to impact human society in the future [17], and nanopesticides were ranked first for their potential low impact on the environment and human health. Benefits such as increased penetration [18], coverage [19] and uptake of active ingredients (AIs) [20] on the target in the presence of NMs will also occur for all agrochemicals with nanotechnology applications in agriculture [21]. Nano-agrochemical delivery system via NMs have great potential for improving the efficacy of agricultural inputs, alleviating environmental pollution, and saving labor costs, which contributes to the maintenance of the sustainability of agricultural systems and the improvement of food security. NMs will have prospective future in agriculture [22].

Fig. 1
figure 1

Nanomaterials loaded with various agrochemicals. Multiple release modes of active ingredients by modulating the structure of the loading system (Particle, Fiber, Layered system, Porous based, Capsule, Emulsion). The small size effect and large specific surface area of nanoparticles can adhere to the target as much as possible to improve the bioavailability of active ingredients

Although NMs have promising applications in agrochemical delivery [23, 24], academics have also raised some concerns [25], mainly in terms of the unknown health risks for human beings and regulatory difficulties brought by the small size effect of NMs. The complexity and cost of manufacturing technology also limit the large-scale use of NMs in agriculture [26]. In this paper, we illustrate the recent advances and challenges of NMs applied as plant protection agrochemicals.

Nanomaterials for pesticide and fertilizer applications

Pesticides and fertilizers are the most widely used agrochemicals in agricultural production [27], and NMs were the earliest to be applied to these two agrochemicals which are also the most researched and reported. Constructing pesticides and fertilizers delivery system through different strategies could improve their utilization and reduce the run-off to environment, which contributes to alleviating environmental pollution and negative impact on the non-target organism caused by excessive use of agricultural inputs.

Nanomaterials for the establishment of pesticide formulations

Pesticides play a crucial role in defending against biological disasters and promoting crop productivity. Most AIs of pesticides are lipid-soluble [28]. Preparing AIs into liquid formulations requires numerous organic solvents; emulsifiers and other additives are also essential. These requirements can trigger overuse of organic solvents, posing a threat to the environment and human health. Solid formulations need solid emulsifiers and many inert materials as fillers, and they are widely used due to their easy storage and transportation. However, dust pollution is caused in the process of production and application, because of lacking effective protection measures, which is prone to harming non-target organisms as well. Wettable powder (WP) and emulsifiable concentrate (EC) are main pesticide formulations in conventional pesticide products [29, 30], but their actual AIs utilization of biological target uptake is less than 0.1% after dust drift and rainwater leaching [31].

NMs used in pesticide production mainly participate in establishing nanopesticide formulation systems to improve the bioavailability of AIs (Fig. 1), detailed reasons as follows (Fig. 2).

Fig. 2
figure 2

Nanomaterials loaded with pesticides. NMs loaded with pesticides can reduce the degradation of AIs caused by environmental influences, achieve target-controlled release of AIs by the properties of nanomaterials, thus prolonging the shelf life of pesticides; Some NMs have their own insecticidal and fungicidal activities; When applied to soil, NMs help pesticides to be fixed in the soil for plant root absorption in order to reduce the loss of pesticides by leaching

NMs improve the stability of AIs. AIs that easily undergo photolysis and are easily decomposed can be stabilized under the protection of NMs. Avermectin (AV) is an excellent bio-pesticide that has been widely used, but it is sensitive to ultraviolet (UV) light and has a very short half-life (6 h) [32]. Zhu et al. grafted AV onto the long chain of poly (ethylene glycol)-carboxymethyl cellulose (PEG-CMC), and the obtained nanoparticles improved the stability of AV significantly over the control, and the degradation rate of AV was less than 50% after 72 h of illumination [33]. Another approach is using NMs as a protective shell for AIs. Kaziem et al. encapsulated AV in hollow mesoporous silica, then anchored cyclodextrin in the outermost layer of the particles [34]. AV was well protected, as less than 9% of AV in these nanoparticles was photolyzed after 24 h UV radiation. Similar to AV, some unstable pesticides such as phoxim, fosthiazate, and quinalphos would likely also be protected by using NMs. Other NMs including lignin and carbon-based materials can also be used as a shell material to protect AIs [35; 36].

NMs can be used for targeted and controlled release of AIs at the optimum working concentration. This prevents waste of AIs and accidental harm to non-targets, and reduces the risk of resistance caused by concentrations of AIs that are too high or too low [37]. Generally, these materials are nanoscale polymers generated by reactions under specific conditions, such as urea–formaldehyde resin [38], polyurethane [39] as well as polyurea [40]. Xiang et al. prepared a magnetic nanocarrier consisting of diatomite and Fe3O4 by in situ deposition. In this formulation, both herbicide (glyphosate) and insecticide (cypermethrin) were wrapped, then coated with chitosan, which could be dissolved to control the release of the pesticide under acidic conditions. The magnetic properties of Fe3O4 help separate nanocarriers from soil and water, facilitating recycling of the materials [41].

Shan et al. used sulfite ions as ring-opening reagents to convert epoxy groups into sulfonic acid groups, and introduced sulfonic acid groups on mesoporous silica nanoparticles (MSNs) by post-grafting to easily fabricate negatively charged decorated MSN-SO3. Diquat dibromide (DQ), the positively charged herbicide was effectively loaded by electrostatic interaction in these negatively charged MSN-SO3 nanoparticles. The loading rate increased to 12.73% for MSN-SO3 compared to unmodified MSN alone as a carrier (5.31%). The release of DQ from DQ@MSN-SO3 nanoparticles was dependent on pH and ionic strength, which were mainly determined by electrostatic interactions. Bioactivity studies showed that DQ@MSN-SO3 nanoparticles exhibited good herbicidal activity in controlling Datura stramonium L. compared to the control [42]. Xiang et al. obtained a pH-reacting controlled-release formulation using attapulgite-based hydrogel. Chlorpyrifos (CPF) was adsorbed to the nano-mesh structure polydopamine modified attapulgite (PA) through hydrogen bonding and electrostatic interaction to obtain CPF-PA. CPF-PA and calcium alginate (CA) subsequently formed porous hydrogel spheres of CPF-PA-CA via a cross-linked reaction, where PA was the skeleton. Under alkaline conditions, the outer layer of material disintegrated and the AIs were released. With the protection of the material, the utilization rate of the AIs was improved and the duration of pesticide activity was prolonged [43].

NMs could decrease the toxicity of pesticides to the non-targets by taking advantage of the isolation effect of the material on the AIs and the organism, which are helpful for enlarging the applicable area of pesticides. Pyraclostrobin has shown excellent control of rice blast disease. This chemical triggers physiological changes in crops, improving their tolerance to the disease and thus significantly increasing yields [44]. However, this compound is highly toxic to aquatic organisms, limiting its application to rice crops [45]. Based on this situation, Li et al. prepared and obtained pyraclostrobin-loaded polyurea nano microcapsules through an interfacial polymerization reaction between isocyanates and amines. The particle size distribution ranged from 815 to 867 nm, forming nearly spherical shapes. This method will reduce pesticide toxicity to aquatic species by coating pyraclostrobin, contributing to improved environmental safety and allowing pyraclostrobin to be applied to rice [46]. Chi et al. developed a core–shell structured magnetic-responsive controlled-release herbicide by a nanocomposite including palygorskite, ferroferrie oxide, glyphosate and amino silicon oil. The release of glyphosate could be accelerated under a magnetic field, and the incomplete coating of amino silicon oil could effectively prevent the herbicide from being completely exposed to the environment and reduce the loss. Biosafety experiment to zebrafish showed that this delivery system had better biosafety than glyphosate [47].

NMs could be directly used as nanopesticides due to their antibacterial or insecticidal properties. NMs can easily penetrate cell membranes due to their nanostructure and small size, and the surface properties of small materials allow them to attach to cell organelles, influencing normal cell organ function and directly or indirectly generating abnormal reactive oxygen species (ROS) [48]. In important physiological processes such as cell signaling, gene expression, and protein redox regulation, a normal ROS level is essential [49]; however, high ROS levels in the above cellular processes cause anomalies and interrupt normal functioning, resulting in cell death [50].

Nanosilver, for example, has biotoxic properties. Bharani and Namasivayam synthesized nanosilver with good control effect against Spodoptera litura [51]. According to the research of Meng et al., the results of in vitro studies showed that silver nanoparticles enter cells through phagocytosis [52, 53] or by passive diffusion [54]. When nanosilver enters the cytoplasm, they appear in intracellular vesicles and can enter mitochondria, nuclei or other organelles [55]. The toxicity of nanosilver depends on particle size. Tang and Zheng summarized the research on the relationship between silver nanoparticles size and its antibacterial properties, and they concluded that the size of silver nanoparticles is a key factor in their antibacterial activity, silver nanoparticles with an average size of 15 nm showed better antibacterial activity against E. coli than the silver nanoparticles with an average size of 75 nm [56]. Shafie et al. [57] used five different concentrations (25, 50, 100, 150 and 200 mg/L) of silver nanoparticles as antiviral agents to reduce tomato spotted wilt virus (TSWV) infestation on Ch. amaranticolor and potato plants. As a result, the different concentrations of silver nanoparticles significantly inhibited the number of local lesions produced by the leaves of Ch. amaranticolor inoculated with TSWV. The inhibition effect was significant (90.4%) after 24 h of spraying silver nanoparticles.

NMs have small size and high specific surface area effect. NMs as carriers increase the solubility of AIs while protecting them from volatilization and degradation [58]. Furthermore, the particle size of pesticide influences the utilization of pesticides [59]. Based on the small size effect and high specific surface area of NMs, more nanoparticles containing AIs of pesticide can be attached to the target per unit area, significantly increasing the area where AIs contact the target and increasing the bioavailability of the AIs [60]. Liu et al. used polylactic acid (PLA) to coat the Lambda–Cyhalothrin, and through the control of process parameters, various drug delivery systems with adjustable particle size between 0.68 μm and 4.6 μm were produced, and they found that the microencapsulated drug delivery systems had good water dispersion and obvious slow-release properties, and the 0.68 μm microcapsules had good biological activity and UV and thermal stability [61]. Wang et al. similarly used PLA to investigate the construction of different particle sizes of AV nano-delivery systems and evaluated their performance. The release rate of AV in the nano-delivery system could be effectively controlled by varying particle sizes, and the results showed that the biological activity was enhanced with decreasing particle size [62].

Generally speaking, the benefits of NMs in delivering Als are obvious, such as improving the stability, increasing utilization rate and bioactivity, and extending shelf life. Studies have shown that the release of Als from delivery systems could be regulated according to the properties of delivery materials and application scenarios. One case in point is pH-sensitive release. Li et al. constructed pH-sensitive thiamethoxam nanoparticles through free radical polymerization and found that the rupture of these nanoparticles in the alkaline intestinal environment of peach aphid could achieve the regulated release of Als [63]. Similar modes include enzyme-sensitive release [64], temperature-sensitive release [65], etc. Usually, establishing indoor release curves is the most commonly used method to determine the sustained-release model of Als, and the release experiments are usually carried out in water or a mixture of water and water-soluble organic solvents [66]. However, it is obvious that the release environment simulated in the laboratory is rather simple, while the real-world environment for release is complex and changeable, as pH, salinity, light, microorganism, and other factors may exert individual or interactive influences on the release of pesticides. Therefore, it is essential to examine the correlation between laboratory-based release model and real-world release model, so as to establish a more accurate and intelligent pesticide delivery system through laboratory studies. This, of course, is still challenging and further studies are needed.

Nanomaterials for fertilizer applications

Currently, chemical fertilizers are favored by farmers [67], because they are more effective and economical than other fertilizers. However, chemical fertilizers can be overused and even wasted, causing soil damage, food yield reduction, and environmental pollution (Fig. 3.). For example, eighty percent of plant demand for N-fertilizers is fulfilled by urea, which is extremely water-soluble and prone to loss [68]. If any N compound is applied to a paddy field, then liquidation, denitrification, volatilization, and runoff cause leaching. Leaching accounts for about 30% to 50% of total N loss, primarily as nitrate, denitrification, about 10–30% as N2, volatilization, and about 2–30% as ammonia [69]. Escaped nitrogen pollutes air and water systems, causing eutrophication. The eutrophication of water contributes to hypoxia and results in many environmental concerns, including mortality of marine animals and algal blooms, and poses a threat to drinking water supplies.

Fig. 3
figure 3

Nanomaterials loaded with fertilizers. Nanomaterial loading of fertilizer. Loading fertilizer with nanomaterials can achieve slow release of fertilizer and improve fertilizer utilization, thus reducing the environmental problems caused by fertilizer loss. In soil, fertilizers were fixed by NMs to facilitate plant roots absorption, reduce the leaching loss of fertilizer, and minimize the pollution of groundwater

As shown in Table 1, NMs have been used in various fertilizers. They are fabricated as controllable and tunable fertilizers and applied to improve fertilizer usage. These formulations enhance nutrient absorption by optimizing soil nutrient management, helping to create a nutrient cycle in agriculture, relieving depletion of nutrient resources, and minimizing the nutrient disordered effect on crop output and the environment. NM-mediated nano-fertilizers can also be used in precision agriculture, with specialist polymers or methods, activated by signals secreted in the environment by plant roots [70]. NMs now are specifically applied as bio-fertilizer; trace element fertilizers such as Fe and Zn; major element fertilizers, including N, P, and K; medium element fertilizers, for example, Si, Ca, Mg, and S. NMs are also used in organic fertilizer. In this section, we present an overview of the application of NMs for each of the fertilizer types as above.

Table 1 Nanomaterials applied to fertilizer and their functions

Djaya et al. developed a composite embedding process for graphite and silica composites to encapsulate the plant growth promoting rhizobacteria (PGPR) and endophytic bacteria to obtain a novel biocontrol delivery system (BDS) [71]. The efficacy of the BDS was evaluated by measuring the diameter of inhibition zone against R. solanacearum culture. The results showed that compared to control, inhibition zone of the treatment of Lysinibacillus sp. was up to 10.24 times wider, and treatment of Bacillus sp. was 12.6 times respectively, which indicated the ability of BDS to form synergistic effect and inducing antagonistic interactions.

Benzon et al. found that compared to conventional fertilizer use only, combining nano-fertilizer with conventional fertilizer significantly improved the agronomic parameters of rice [72]. The plant height, chlorophyll content, number of reproductive tillers, panicles and spikelets were increased by 3.6%, 2.72%, 9.10%, 9.10% and 15.42%, respectively. Panicle weight, total grain weight, total shoot dry weight and harvest index were also improved by 17.4%, 20.7%, 15.3%, and 2.9%, respectively. Furthermore, the authors determined that the performance of using only nano-fertilizer was not noticeable and suggested using nano-fertilizer in conjunction with conventional fertilizer to achieve good results.

Haydar et al. evaluated the biological effects of iron oxide nanoparticles as well as EDTA-functionalized iron oxide nanoparticles as a nano-micronutrient fertilizer in place of conventional iron fertilizers [73]. The effect of these fertilizers on agronomic trait parameters of mulberry (Morus alba L.) was estimated in a pot experiment. The application was carried out by both foliar spraying and soil application, and iron oxide nanoparticles and their EDTA functionalized forms were applied at two different concentrations (10 and 50 mg/kg soil). The results showed positive effects on the agronomic trait parameters of the treated plants. Iron oxide nanoparticles in the soil at a concentration of 10 mg/kg significantly improved the morphological characteristics of the plants, such as germination rate, leaf number (52.73% higher than the control), plant biomass (37.20% and 90.24% increase in shoot and root biomass, respectively, compared to the control), root properties (34% increase in root length), and also reduced the time to germination of the first leaves. The same treatments increased by 42% and 15%, respectively, compared to the control for chlorophyll and sugar content. The activity of antioxidant enzymes such as catalase, peroxidase and NADPH oxidase was also increased compared to the control. All results showed that iron oxide nanoparticles could replace the traditional iron fertilizer in the cultivation and propagation of mulberry crops.

Raliya et al. developed nano zinc fertilizer to increase the yield of pearl millet (Pennisetum americanum) [74]. In this study, the fungus Rhizoctonia bataticola TFR-6 (NCBI GenBank Accession number JQ675307) was cultivated in potato dextrose (PD) broth medium. The filtrate of fungus-free organism cells was isolated, which was used to prepare an aqueous zinc oxide solution with a concentration of 0.1 mM. The synthesis of nano zinc was performed by exposing the former zinc oxide solution to the fungal cell-free filtrate previously prepared for 62 h. The dynamic light scattering (DLS) results showed that the particle size of the synthesized nanoparticles ranged from 15 to 25 nm and had spherical with clear particle interfaces and crystalline structures as seen by TEM. Fertilization with the nano-fertilizer applied to 6-week-old pearl millet showed the following results: stem length (15.1%), root length (4.2%), root area (24.2%), chlorophyll content (24.4%), total soluble leaf protein (38.7%), plant dry biomass (12.5%), acid phosphatase (76.9%), alkaline phosphatase (61.7%), phytase (322.2%) and dehydrogenase (21%) activities were significantly higher than the control. The application of Zn nano-fertilizer increased the yield of the crop at maturity by 37.7%.

Li et al. found via field and indoor experiments that the long-term use of pesticides in tea gardens would cause the plants to accumulate excessive amounts of ROS, leading to oxidative damage in the plants and affecting the nutritional quality of tea [75]. In contrast, selenium nanoparticles activated the plant antioxidant system, resulting in significant increases in the content and activity of antioxidant enzymes in the ascorbic acid-glutathione cycle, which helped minimize pesticide induced oxidative damage. Biofortification with selenium significantly increased the accumulation of nutrients and functional substances such as proteins, carotenoids, tea polyphenols, and catechins in tea products. Metabolomic and transcriptomic studies have shown that selenium nanoparticles can induce theanine, glutamate, proline, and arginine production by regulating the glutamine-glutamate cycle. Nanoselenium exogenous supplementation promotes secondary metabolism in tea plants, which in turn increases the biosynthesis of total phenols and flavonoids in tea leaves. Nanoselenium promotes the uptake and conversion of selenium in the soil, leading to a significant increase in the production of selenium-rich teas with many key aroma components.

Alimohammadi et al. evaluated the effect of urea and Nano-Nitrogen Chelate (NNC) fertilizers on yield of sugarcane (Saccharum Officinarum) and nitrate leaching from soil [76]. Treatments included five different N contents of urea and NNC (0, 80, 112, 137 and 161 kg N ha−1). Results showed that the mean values of soil nitrate concentrations were 10.2 and 12.8 mg/kg for urea and NNC treatments, respectively, during sugarcane growth. The nitrate leaching level was high with urea fertilizer (699.0 mg l−1) and low with NNC fertilizer (183.0 mg l−1). This study also found that the application of NNC was significantly effective in increasing sugar content in sugarcane. Considering nitrate leaching and its impact on human health and the environment, NNC is potentially valuable.

Rajonee et al. prepared phosphorus and potassium compound nano-fertilizer using zeolite as a carrier material, and characterized and evaluated fertilization efficiency [77]. Material composition measured by X-ray diffraction (XRD) showed that the modified zeolite's peak position and height changed compared with unmodified zeolite, indicating that the K, P fertilizer element was successfully loaded into the zeolite and the nano-fertilizer was prepared successfully. An in vitro incubation study with the application of nano-fertilizer showed that the release of potassium was most prominent throughout the experiment and all experimental units showed the same trend to different degrees. The potassium content in the soil was maintained at a higher level with the nano-potassium fertilizer compared to control. Even though nano-fertilizer has promising performance, its large-scale implementation is limited by high cost.

Chen et al. revealed that CaSO4 reduced phosphorus loss from farmland through soil incubation experiments [78]. CaSO4 is a typical soil conditioner with strong complexation to orthophosphate. Compared to conventional crude CaSO4, nano calcium sulfate provides a larger surface area, higher solubility, and improved integration of fertilizer and soil, which further reduces soil phosphorus loss.

Ahanger et al. presented a nano-vermicompost organic fertilizer with particle sizes ranging from 1 µm to 200 nm after air-drying and grinding [79]. The influence of this fertilizer on the growth of tomatoes under drought stress was studied, and the results showed beneficial effects on growth, oxidative stress parameters, antioxidant, nitrogen metabolism, osmotic accumulation and mineral elements under drought stress, which demonstrated its beneficial contribution in preventing drought-mediated damage to tomatoes.

Currently, NMs in fertilizer are mostly reported from validation experiments illustrating that NMs can be used as a fertilizer directly or as auxiliary materials to indirectly improve fertilizer utilization. The lack of systematic theoretical studies on interactions and material exchange among NMs, plant root systems, root physical and microbial environments, and plant systems hinders the development and implementation of nano-fertilizer formulations.

The reported mechanisms by which NMs act as fertilizer or fertilizer synergists to affect plant growth can be summarized as the following aspects: First, reducing fertilizer particle size to nanoscale is expected to improve the solubility and dispersion potential of fertilizer nutrients. Furthermore, due to the small size effect of NMs, they have unique potential to easily pass through the membrane barriers of plant cell walls, thereby offering altered uptake kinetics of the applied nano-form fertilizers [80]. Lastly, NMs protect fertilizer by encapsulating it to reduce its volatilization, fixing it in the soil through NMs and reducing its leaching losses, thus improving its utilization and reducing environmental damage. NMs and nanotechnology in fertilizers will effectively promote fertilizers' absorption and utilization, thus improving the agronomic parameters of crops. It can also reduce the environmental pollution caused by the volatilization and leaching of chemical fertilizers.

New applications of nanomaterials in agrochemicals

In recent years, because of the environmentally friendly control strategies advocated by Integrated Pest Management, the rapid rise of organic farming, and the increased awareness of environmental protection and food safety, many eco-friendly agrochemicals, such as bio-pesticides have received lots of attention, NMs are subsequently applied to these chemicals [81].

Nanomaterials for bio-pesticide applications

The ecological and health threats associated with applying chemical pesticides are increasingly concerning. According to the reports of Tang and Tackenberg et al., in the past few years, bee numbers have decreased markedly and trace pesticides have been detected in 75% of the world's honey, particularly neonicotinoids, including imidacloprid, thiacloprid, and thiamethoxam. With this background, some environmentally-friendly agricultural solutions for pest control that pose little risk to humans and beneficial microorganisms have gained more attention [82, 83].

Compared with chemical pesticides, bio-pesticides are more eco-friendly, and they have become more common for pest and disease control in plants. Currently, the market share of bio-pesticides is increasing, with the following categories [84].

  1. (1)

    Microbial pesticides, developed using living microorganisms, specifically subdivided into bacterial pesticides, such as Bacillus thuringiensis (Bt), B. subtilis, and B. cereus; and fungal pesticides, such as Beauveria bassiana (Bals.), Metarhizium anisopliae, (Paecilomyces lilacinus (Thom.) Samson), and Verticillium.

  2. (2)

    Viral pesticides, including nuclear polyhedrosis virus (NPV), granulosa virus (GV), and genetically engineered rod-shaped virus.

  3. (3)

    Plant-derived pesticides are natural products in plants, some of which have insecticidal and fungicidal effects, such as EOs, bittersweet, pyrethrins, indocyanin.

  4. (4)

    Agricultural antibiotics are microbial secondary metabolites produced by bacteria, fungi, actinomycetes, and other microorganisms in the fermentation process, which can be used to control agricultural pests. Examples include AV, bupropion, erythromycin, and liuyangmycin.

  5. (5)

    Biochemical pesticides, such as vinblastine, erythromycin, and gibberellic acid.

  6. (6)

    Plant immunity elicitor-inducing antibacterial agents, which allow plants to fight against pests and diseases by enhancing their immune function, such as chitosan and activating proteins.

Bio-pesticides also include natural enemy pesticides, such as Trichogramma dendrolimi (Matsumura) and T. chilonis (Ishii).

However, bio-pesticides have their own drawbacks. For example, microbial pesticides and antibiotic pesticides are easily affected by external environment factors such as temperature, humidity, and light, making the AIs unstable. Using NMs as carriers is one way to solve the stability and utilization issues of bio-pesticides effectively (Table 2).

Table 2 Nanomaterials applied to bio-pesticide and their functions

When Bt forms spores accompanied by the production of insecticidal crystal protein, which is a protein of length 130–140 kDa, it needs to be solubilized in an environment with a pH > 9.5 to be activated and then become toxic. The midgut of lepidopteran larvae is a strong alkaline environment, and when the larvae intake Bt, it is activated and releases 60–70 kDa of toxin, killing the target. Therefore, the smaller the particle size of Bt, the better its performance. Usually, top-down approaches of microionization are employed to make Bt in the nanoform. These approaches, including jet milling, ball milling, and high pressure homogenization, could convert the coarse powder to ultrafine powder, and are easy to scale up and give reproducible results [85]. However, the balls used in the milling may produce contamination of the final product, and heat generation during high speed milling process could reduce the viability and efficacy of Bt. Therefore, an increase of using NMs to load Bt is observed. The preparation of NMs loaded Bt could not only obtain ultrafine formulations, but also improve the stability and shelf life of biological pesticides in the environment. Zaki et al. studied the application of NMs in Bt formulations and investigated a complex of nanotubular sodium titanate and Bt as a novel nano-pesticide against cotton leaf-worm. The preparation was carried out by mixing the NMs sodium titanate and Bt in water at a mass ratio of 1:2, sonicating for 10 min, then stirring with a magnetic stirrer, and finally drying [86]. The bacterial nanocomposites showed an improvement in insecticidal activity against cotton leaf worm, Spodoptera littoralis and a stronger effect on the different biological features of pest than bacteria alone.

In order to address the poor stability and low utilization of Bt, excipients were also added to prevent settling of suspensions; preservatives were added to extend shelf life; and for powders, additives were applied to improve their wetting and spreading properties. UV shielding agents were also used to prevent rapid photolysis after spraying. These improvements resulted in a sixfold increase in the utilization of AIs [87].

Beauveria bassiana (Bals.) is a fungal microbial pesticide, usually prepared with vegetable oil and mineral oil as dispersion media to obtain oil suspensions for use. B. bassiana is susceptible to decomposition by UV irradiation, so Hersanti et al. used silica nanoparticles (Silica Nps.) and carbon fibers as carriers for B. bassiana to control potato Spodoptera litura (S. litura) [88]. Silica Nps. and carbon fibers can also be used as fertilizers to provide nutrients to plants while protecting them from biological stress. A total of 16 treatments (control treatments were included) were designed to assess the effect of applying different concentrations of the mixture B. bassiana + Silica Nps. + carbon fiber on the mortality of S. litura. The results showed that the application of the mixture of B. bassiana + Silica Nps. 5% + carbon fiber had the highest mortality rate (53.33%) on S. litura larvae, while the single B. bassiana treatment had a mortality rate of 6.67%.

Plant-derived pesticides are also important bio-pesticides, and currently, EOs and neem-derived products are the most widely used [89]. EOs, an important natural source of pesticides, are mostly lipophilic and interfere with insects' normal metabolic, biochemical, and physiological reactions, which eventually affect their behavior. NMs applied to plant EOs show a synergistic effect on EO bioactivity. Cinteza et al. mixed chitosan-coated nanosilver with EOs in specific ratios, and the physical and chemical properties and biological activity of the system remained stable after mixing. In addition, a synergistic effect from the antibacterial activity test was observed, and the mixture was effective against a wide range of microorganisms with minimal side effects [90].

NMs are applied in antibiotic bio-pesticides to raise the stability and utilization of AIs. AV is the most widely used antibiotic-based bio-pesticide. As noted earlier, NMs improved the anti-UV ability of AV, increased the biocompatibility of the AIs, and improved its utilization.

In addition, microbial pesticides and chemical pesticides can be loaded together through NMs to form a dual-functionalized system, which has a synergistic effect on the bioactivity. Cui et al. used water–oil-water double emulsion method combined with high-pressure homogenization technology to prepare PLA nanocapsules containing validamycin and thifluzamide (VTNC) for the control of rice sheath blight [91]. The VTNC shown an average diameter of 265.3 ± 0.8 nm and polydispersion index of 0.034 ± 0.030, and these spherical particles were well dispersed, smooth in surface, and equal in size. The bioactivity evaluation results of VTNC against Rhizoctonia solani. showed that dual-functionalized system was significantly better than that of the commercial formulations and a clear synergistic effect between the two AIs was observed.

Gibberellic acid (GA) is an important biochemical pesticide, and is relatively widely used. GA stimulates plant growth, such as promoting the formation of male flowers [92]. Hafez et al. used layered double hydroxides (LDH) as a potential carrier for a GA formulation [93]. GA@LDH nano-formulation was synthesized using the memory-effect property of LDH. TEM showed a hexagonal flake-like shape with a lateral dimension ranging from 10 to 20 nm. The GA@LDH nano-formulation promoted plant growth with a significant increase in stem length over the control at the same concentration, and also showed better bioavailability and improved safety compared to the control. This study highlights a potential nanohybrid formulation form of GA using the memory effect property of the LDH clay material.

As a plant immunity elicitor, chitosan stimulates plant growth, induces disease resistance in plants, and produces immunity and inactivation against various fungi, bacteria, and viruses [94]. Asgari-Targhi et al. synthesized chitosan/zinc oxide nanocomposite (CS-ZnONP) and evaluated their biologically in vitro conditions [95]. The nanocomposite was able to improve plant growth and biomass more than bare ZnONPs use. Plants with CS-ZnONP increased chlorophyll (51%), carotenoids (70%), proline (twofold) and protein (about twofold) concentrations than with bare ZnONPs. Adding CS-ZnONP to the seed medium increased enzymatic antioxidant biomarkers (catalase and peroxidase). The activity of phenylalanine ammonia-lyase showed a similar significant upward trend. CS-ZnONP treatment greatly enhanced the accumulation of alkaloids (60.5%) and soluble phenols (40%). Micropropagation experiments showed that CS-ZnONP treatment promoted organ development. Overall, CS-ZnONP can be considered as a highly effective biocompatibility elicitor.

The integrated system of pesticides and fertilizers constructed with NMs and nanotechnology can achieve the co-delivery of pesticides and fertilizers, which is of great potential in reducing labor cost and giving full play to the synergistic effects of fertilizers and pesticides [96].

Nanomaterial-mediated nucleic acid pesticides system

Nucleic acid pesticides are based on the introduction of exogenous nucleic acid fragments into the target, which interferes with the normal transcription of the target gene, resulting in the death of the target. The more studied are RNA pesticides. RNA pesticides are used to protect crops with RNA interference (RNAi) technology. RNAi delivers double-stranded (ds)RNA /small interfering (si) RNA to insects, silencing genes and affecting normal genetic coding, ultimately killing the pests (Fig. 4.) [97]. RNA-pesticides work through the initiation, effect, and cascade amplification of RNAi by Dicer-like (DCL), Argonaute(AGO), and RNA-dependent RNA polymerase (RdRP). The RNA-induced silencing complexes (RISC) assembled by functional small RNAs and AGO enzymes are the minimal functional units of gene silencing [98]. DCL participates in cleavage and processing of small RNA precursors (dsRNA or microRNA precursors, etc.) to form mature small RNA sequences [99]. AGO is implicated in the recognition and selective binding of small RNAs to promote the formation of functional RISC complexes and mediate target sequence degradation or translational repression [100]. RdRP is mainly associated with the generation and replication of secondary small RNAs, further enhancing and amplifying the silencing effect [101]. Therefore, functional small RNAs are the core elements of biologically mediated RNAi-dependent gene silencing, and DCL, AGO, and RdRP are critical for maintaining the generation, transmission, and amplification of RNAi-mediated gene silencing signals [102].

Fig. 4
figure 4

Nanomaterial-mediated nucleic acid pesticides system. NMs protect nucleic acids, reduce degradation and help them enter the cell of target pests. Then, the RNAi program is initiated to interfere with the normal transcriptional pathway of the target and achieve the effective control of the pests

RNA pesticides are safe and environmentally friendly because of their strong specificity, low development cost, and safety. These pesticides meet current criteria for sustainable agriculture, as a potential environmentally friendly and effective pesticide for plant protection. However, RNA pesticides are unstable, easily degraded, and decompose themselves before reaching the target. NMs are applied in RNAi delivery systems in order to protect siRNA and improve its efficiency for entering into pests [103].

Mitter et al. demonstrated the possibility of using LDH-bioclay as a carrier for transporting dsRNA applied to crop protection, as an alternative to the traditional transgenic treatment method [104]. LDH-bioclay firmly adhered to the leaf surface of Vigna unguiculata ssp. unguiculata (cowpea) and Nicotiana tabacum cv. Xanthi nc even after vigorous rinsing. Longer stability of dsRNA protected by LDH-bioclay was ensured under the environmental conditions of application, with a persistence period of up to 20 days. The sustained release of dsRNA was facilitated by the formation of carbonic acid on the leaf surface from atmospheric CO2 and H2O.

NMs also serve as DNA nanocarriers. Kwak et al. demonstrated the possibility of using single-wall chitosan-complex carbon nanotubes for chloroplast transformation [105]. They prepared single-wall carbon nanotube carriers (SWNT) based on chitosan to secure plasmid DNA (pDNA) and deliver it to the chloroplast. Chitosan-modified SWNT was rationally designed using the lipid exchange envelope penetration model to maximize the efficiency of carrying the pDNA-SWNT complex into chloroplasts. By using pDNA encoding a yellow fluorescence protein reporter gene, Kwak et al. demonstrated chloroplast-targeted gene delivery and transgene expression in mature arugula, dulse, spinach, and tobacco plants as well as isolated Arabidopsis thaliana protoplasts.

Demirer et al. noted that high aspect ratio carbon nanotubes enable delivery of functional genetic material without DNA integration in mature plants [106]. Therefore, this delivery system could achieve efficient genome modification without transgene integration, thus avoiding strict genetically modified organisms regulations. Moreover, this work shows that carbon nanotubes can protect DNA cargo from nuclease degradation. Such studies showed the potential of delivering biomolecules into plant cells through DNA nanostructures, and DNA nanocarriers provide important guidance for the development of effective ligand-mediated nucleic acid delivery systems.

Nanomaterials applied in plant growth regulators

Plant growth regulators (PGRs) are a special family of pesticides mainly manufactured by artificial synthesis and from substances with high biological activity. They regulate the growth and development of plants at very low concentrations. Similar in concept to PGRs are the five major categories of endogenous plant hormones, namely auxin (IAA), gibberellins (GA), cytokinins (CTK), abscisic acid (ABA), and ethylene (ETH). PGRs and plant hormones have the same chemical structure and biological effects, only the derivation is different [107]. NMs in combination with PGRs are mainly used to help detect trace amounts of plant hormones in plants and flexibly adjust the hormone levels to obtain the best production value. Moreover, NMs assist in the absorption and transport of PGRs into the plant.

Chen successfully prepared β-Cyclodextrin modified magnetic graphene oxide material (Fe3O4@SiO2/GO/β-Cd) [108]. This is a novel magnetic sorbent for the enrichment and purification of PGRs in plants before GC–MS detection. The advantages of this material include its magnetic separation properties, large surface area due to its small size, high supramolecular recognition rate, low cost, and environmental friendliness. Using this material, accurate test results were obtained for trace levels of five PGRs in real vegetable samples, with good linear relationships in the range of 1–100 lg/kg with determination coefficients (R2) from 0.9983 to 0.9996. Similarly, Li et al. used crystalline porous polymer material to prepare a sorbent for PGR detection [109]. They chose covalent organic frameworks (COFs) to synthesize magnetic covalent organic frameworks Fe3O4@COF(TpDA), which were constructed with organic building units via covalent bonds. The results showed good linearity (R ≥ 0.9990) and low detection limits (4.68–7.51 μg/L) with a recovery of 83.0%-105.0%, suggesting that this method can be used to detect PGRs in fruits and vegetables.

Santo Pereira et al. developed alginate/chitosan (ALG/CS) and chitosan/ tripolyphosphate (CS/TPP) nanocarriers to encapsulate the PGR GA-3 [110]. ALG/CS nanoparticles with and without GA-3 had a mean size of 450 ± 10 nm, PDI of 0.3, and a zeta potential of + 27 ± 3 mV. ALG/CS-GA-3 nanoparticles with 100% encapsulation efficiency and bioactivity results showed an increase in leaf area, chlorophyll, and carotenoid content compared to the control.

NMs themselves can also regulate the growth and development of plants. Khodakovskaya et al. discovered that multi-walled carbon nanotubes (CNTs) influence plant growth and the composition of soil microorganisms [111]. CNTs were applied to tomato cultivation and there were twice as many fruits and flowers in tomatoes with CNTs than in the control. In a study of soil microbial communities, the relative abundances of Bacteroidetes and Firmicutes increased, whereas Proteobacteria and Verrucomicrobia decreased with increasing concentrations of CNTs. Chakravarty et al. used graphene quantum dots as enhanced PGRs to validate their effects on coriander and garlic growth [112]. The results showed that graphene quantum dots used to treat seeds promoted the growth rate of coriander and garlic plants, including leaves, roots, shoots, flowers and fruits. Graphene quantum dots can be used in a variety of other plants for high production.

Nanomaterials applied in pheromones

Attract-and-kill through pheromones is one of the most potent approaches to integrated pest management [113], and continuous release of pheromone active substances is required during the pest capture period (Fig. 5.). However, due to the volatile nature of pheromones, their duration is usually very short, and frequent replacements are needed during field application. This is a major disadvantage of pheromones that needs to be solved. Larson et al. developed a controlled release polyethylene dispenser for the controlled release of pheromone AIs [114]. Seo et al. used cetyltrimethylammonium to modify A zeolite and synthesized a pheromone distributor, which has a low Si/Al ratio with nanoporous structure and possesses a high cation exchange capacity to maximize the adsorption of pheromone components [115]. Attraction test showed that the nanoporous carrier exhibited better elicitation performance than commercial product under the same environment. Correia et al. also developed a composite membrane for the slow release of pheromones using poly (butylene adipate‑co‑terephthalate) and activated charcoal, which could protect the AI from climatic factors and prolong the shelf life of the pheromone [116]. The results of diffusion experiment showed that the volatility of pheromone rhynchophorol was 100% within 24 days for the control sample, while the volatility of composite membrane-coated rhynchophorol was only 45% after 65 days.

Fig. 5
figure 5

Nanomaterial loaded pheromone. Traditional pheromones products are unstable and easy to volatilize, resulting in short shelf life and unsatisfactory "Attract-and-kill" effect; Nanomaterial-loaded pheromones with environment friendly properties can realize slow-release, protect from decomposition under ambient conditions, and show excellent efficacy in the open orchard

Bhagat et al. prepared pheromone methyl eugenol (ME) nanogels using low-molecular mass gelators (LMMGs)-all-trans tri (p-phenylene ethynylene) bis-aldoxime [117]. The preparation was based on the self-assembly by weak intermolecular non-covalent interactions (e.g., hydrogen bonding, π-π stacking and van der Waals) of LMMGs, which leads to specific solvent gelation. The gel system was a three-dimensional nanoscale supramolecular network structure, providing high pheromone retention capacity. In addition, the nanogel was insoluble in water, which protected ME from decomposition caused by light, temperature and humidity in the environment, thus prolonged the shelf life. The perpetration did not require the use of any additives, such as crosslinkers and antioxidants, so it was an environmentally friendly agrochemical. The results of field trapping tests showed that in the luring test of B. dorsalis, treatment 1 (ME alone) and treatment 2 (ME in nanogel) were basically equal in the first three days, while from the 8th day, treatment 2 showed a more effective effect than treatment 1. Therefore, nanogel immobilized ME was an effective agent for the control of B. dorsalis.

Future challenges in the development of nano-agrochemicals

NMs are still being explored for applications in agrochemicals, and safety assessment, production cost, evaluation standards, registration polices and public concern issues must be addressed. A critical assessment of the impact of nanoparticles is necessary before they are determined to be safe to use [118].

Environmental safety assessment

There are concerns about the potential safety risks of NMs to non-target organisms as well as to humans. Juárez-Maldonado et al. summarized that the direct interaction of NMs with microbial cells is also quite complex and may cause specific changes in microbial cell physiology and gene expression that can lead to proliferation of any kind of cell [119]; such risks may reduce the diversity and abundance of specific microbial groups in the soil [120]. Risk assessment of NMs for agrochemicals primarily remains in the laboratory stage and the real scenarios of application do not have enough risk assessment. Various application environments may lead to variable results. For the design and predict the environmental fate of the nanoscale agrochemicals, Zhang et al. proposed to integrate existing nutrient cycling and crop productivity models with nanoinformatics approaches to optimize targeting, uptake, delivery, nutrient capture, and long-term effects on soil microbial communities to design nanoscale agrochemicals that combine the best safety and functional properties [121]. Kah et al. suggested that nano-agrochemicals should be extensively tested for effectiveness and environmental effects under field conditions [122]. Further work needs to be done to evaluate nano-agrochemicals to assess the advantages and new risks of existing products in a reasonable manner.

Production cost, evaluation standards and registration policies

Though nano-enabled agrochemicals may offer a range of benefits, they are still in the early developmental stage. Several companies have deposited patents comprising numerous protocols for production and application of nano-products, whereas, the commercial nanotechnology-based products is still relatively limited in quantity and needs further development. According to the Nanotechnology Products Database available at Statistic Bank (StatNano), there are 9420 nanotechnology-based products are commercialized, only 229 nano-products launched for agriculture application [123]. Most of these products are nano-fertilizers (43%), and nanoformulations aimed at improving plant survival against disease, pest and other stress represent together 28% of these products. The high production costs and complexity of the production process make the use of NMs in agriculture a low-margin industry that cannot generate significant economic returns on the initial production investment [124]. This is a major constraint to the application of NMs in agrochemicals on a large scale. Furthermore, there is a lack of uniform methods and standards among government authorities relating to NMs [125]. The existing methods of government regulators are not applicable or cannot correctly evaluate the safety risks of nano-pesticides, and there is an urgent need to study and establish corresponding evaluation methods and standards, for the large-scale application of NMs in agrochemicals. The pesticide registration authorities in the US and European countries have issued registration policies for nano-pesticides. The US EPA has used the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) to require the registration of nano-pesticides as “new” substances and to refine relevant registration policies. The EU member states register pesticides under the Plant Protection Products Regulation, which applies to the registration of single pesticides and mixtures, regardless of size, and this includes the registration of nano-pesticides [126].

The public's acceptance of new technologies also hinders the industrialization of NMs in agriculture to a certain extent, especially as agriculture is closely related to food safety and people are likely to be sensitive to the application NMs in agriculture.

Prospects for nanomaterials applied in agrochemicals

In this review, we discussed the application of NMs and nanotechnology in plant protection, including the use of NMs in constructing nano-pesticide formulation systems, nano-fertilizers, bio-pesticides, nucleic acid pesticides, PGRs, and pheromone. We also noted the challenges of industrial production of NMs for plant protection. Although nano-agrochemical delivery is at an early stage of development, it is expected that this application will improve the efficiency of agrochemicals and reduce environment pollution. More research is required to facilitate their large-scale application. In the future, the research focus of nano-agrochemicals are as follow; (1) competition with the conventional formulations in performance, cost and scale manufacture technology, especially in the prospect of commercialization; (2) establishment of new evaluation standards of nano-agrochemicals on product quality and safety; (3) precise controlled-release systems able to response to the changes in environmental stimuli such as pH, light, temperature, enzyme activity, etc.; (4) establishment of specific using guidelines and effective large-scale field application technology of nano-agrochemicals to facilitate their extension in agriculture.

Industrial production of nano-agrochemicals prepared by NMs and nanotechnology are influenced by many factors including product economics, environmental factors, evaluation standards, using guidelines and policy issues, but the benefits of NMs and nanotechnology applied in agricultural are likely to be monumental because it can improve the efficacy, accuracy and targeting of agrochemicals. In addition, the introduction of reasonable policies and popularization of the new technology among the public are also beneficial to promote its development.

Availability of data and materials

Not applicable.





Plant growth regulators


Nanostructured materials


Engineered nanomaterials


Plant growth regulators


Active ingredients


Wettable powder


Emulsifiable concentrate






Poly (ethylene glycol)-carboxymethyl cellulose






Calcium alginate


Reactive oxygen species


Polylactic acid


Sodium bentonite


Essential oils


Transmission electron microscopy


Potato dextrose


Dynamic light scattering


X-ray diffraction













Bt :

Bacillus thuringiensis


Beauveria bassiana


Nuclear polyhedrosis virus


Granulosa virus


Porous hollow silica nanoparticles


Gibberellic acid


Layered double hydroxides


Poly (ethylene glycol)-carboxymethyl cellulose


RNA interference


Double-stranded RNA


Small interfering RNA






RNA-dependent RNA polymerase


RNA-induced silencing complexes


Single-wall carbon nanotube carriers


Plasmid DNA








Abscisic acid




Covalent organic frameworks




Chitosan/ tripolyphosphate


Carbon nanotubes


Methyl eugenol


Low-molecular mass gelators


Federal insecticide, fungicide, and rodenticide act


  1. Feynman R. P. There's plenty of room at the bottom. Engineering and science. 1959, p. 23.

  2. Pokropivny VV, Skorokhod VV. Classification of nanostructures by dimensionality and concept of surface forms engineering in nanomaterial science. Mat Sci Eng C-Mater. 2007;27(5–8):990–3.

    Article  CAS  Google Scholar 

  3. Anandhi S. Nano-pesticides in pest management. J Entomol Zool Stud. 2020;8(4):685–90.

    Google Scholar 

  4. Selyutina OY, Khalikov SS, Polyakov NE. Arabinogalactan and glycyrrhizin based nanopesticides as novel delivery systems for plant protection. Environ Sci Pollut Res. 2020;27:5864–72.

    Article  CAS  Google Scholar 

  5. Selyutina OY, Apanasenko IE, Khalikov SS, Polyakov NE. Natural poly-and oligosaccharides as novel delivery systems for plant protection compounds. J Agric Food Chem. 2017;65(31):6582–7.

    Article  CAS  PubMed  Google Scholar 

  6. Roco MC, Williams RS, Alivisatos P. Nanotechnology research directions: IWGN workshop report: vision for nanotechnology in the next decade. Berlin: Springer; 2000.

    Book  Google Scholar 

  7. Scott N, Chen H. Nanoscale science and engineering for agriculture and food systems. Ind Biotechnol. 2013;9(1):17–8.

    Article  Google Scholar 

  8. de Oliveira JL, Campos EVR, Bakshi M, Abhilash PC, Fraceto LF. Application of nanotechnology for the encapsulation of botanical insecticides for sustainable agriculture: prospects and promises. Biotechnol Adv. 2014;32(8):1550–61.

    Article  CAS  PubMed  Google Scholar 

  9. Huang B, Chen F, Shen Y, Qian K, Wang Y, Sun C, Zhao X, Cui B, Gao F, Zeng Z, Cui H. Advances in targeted pesticides with environmentally responsive controlled release by nanotechnology. Nanomaterials. 2018;8(2):102.

    Article  CAS  PubMed Central  Google Scholar 

  10. Kumar V, Vaid K, Bansal SA, Kim KH. Nanomaterial-based immunosensors for ultrasensitive detection of pesticides/herbicides: current status and perspectives. Biosens Bioelectron. 2020;165: 112382.

    Article  CAS  PubMed  Google Scholar 

  11. Mahmoudpour M, Karimzadeh Z, Ebrahimi G, Hasanzadeh M, Ezzati Nazhad Dolatabadi J. Synergizing functional nanomaterials with aptamers based on electrochemical strategies for pesticide detection: current status and perspectives. Crit Rev Anal Chem. 2021.

    Article  PubMed  Google Scholar 

  12. Adams CB, Erickson JE, Bunderson L. A mesoporous silica nanoparticle technology applied in dilute nutrient solution accelerated establishment of zoysiagrass. Agrosyst Geosci Environ. 2020;3(1): e20006.

    Article  Google Scholar 

  13. Taşkın MB, Şahin Ö, Taskin H, Atakol O, Inal A, Gunes A. Effect of synthetic nano-hydroxyapatite as an alternative phosphorus source on growth and phosphorus nutrition of lettuce (Lactuca sativa L.) plant. J Plant Nutr. 2018;41(9):1148–54.

    Article  CAS  Google Scholar 

  14. Fincheira P, Tortella G, Seabra AB, Quiroz A, Diez MC, Rubilar O. Nanotechnology advances for sustainable agriculture: current knowledge and prospects in plant growth modulation and nutrition. Planta. 2021;254(4):1–25.

    Article  CAS  Google Scholar 

  15. Dikbaş N, Cinisli KT. Microbial metabolites powered by nanoparticles could be used as pesticides in future? (NanoBioPecdicides). BJI. 2019;23(4):1–4.

    Article  CAS  Google Scholar 

  16. Lade BD, Gogle DP. Nano-biopesticides: synthesis and applications in plant safety. In: Abd-Elsalam K, Prasad R, editors. Nanobiotechnology applications in plant protection. Nanotechnology in the life sciences. Cham: Springer; 2019.

    Google Scholar 

  17. Ten G-B. chemical innovations that will change our world: IUPAC identifies emerging technologies in chemistry with potential to make our planet more sustainable. Chem Int. 2019;41(2):12–7.

    Article  CAS  Google Scholar 

  18. Giraldo JP, Landry MP, Faltermeier SM, McNicholas TP, Iverson NM, Boghossian AA, Reuel FN, Hilmer JA, Sen F, Brew AJ, Strano MS. Plant nanobionics approach to augment photosynthesis and biochemical sensing. Nat Mater. 2014;13(4):400–8.

    Article  CAS  PubMed  Google Scholar 

  19. Su C, Ji Y, Liu S, Gao S, Cao S, Xu X, Zhou C, Liu Y. Fluorescence-labeled abamectin nanopesticide for comprehensive control of pinewood nematode and Monochamus alternatus hope. ACS Sustain Chem Eng. 2020;8(44):16555–64.

    Article  CAS  Google Scholar 

  20. García-Gómez C, Obrador A, González D, Babín M, Fernández MD. Comparative study of the phytotoxicity of ZnO nanoparticles and Zn accumulation in nine crops grown in a calcareous soil and an acidic soil. Sci Total Environ. 2018;644:770–80.

    Article  CAS  PubMed  Google Scholar 

  21. Li P, Huang Y, Fu C, Jiang SX, Peng W, Jia Y, Peng H, Zhang P, Manzie N, Mitter N, Xu ZP. Eco-friendly biomolecule-nanomaterial hybrids as next-generation agrochemicals for topical delivery. EcoMat. 2021.

    Article  Google Scholar 

  22. Pulizzi F. Nano in the future of crops. Nat Nanotechnol. 2019;14(6):507.

    Article  CAS  PubMed  Google Scholar 

  23. Xu T, Ma C, Aytac Z, Hu X, Ng KW, White JC, Demokritou P. Enhancing agrichemical delivery and seedling development with biodegradable, tunable, biopolymer-based nanofiber seed coatings. ACS Sustain Chem Eng. 2020;8(25):9537–48.

    Article  CAS  Google Scholar 

  24. Guha T, Gopal G, Kundu R, Mukherjee A. Nanocomposites for delivering agrochemicals: a comprehensive review. J Agric Food Chem. 2020;68(12):3691–702.

    Article  CAS  PubMed  Google Scholar 

  25. Malakar A, Kanel SR, Ray C, Snow DD, Nadagouda MN. Nanomaterials in the environment, human exposure pathway, and health effects: A review. Sci Total Environ. 2020.

    Article  PubMed  Google Scholar 

  26. Hussain CM. Handbook of nanomaterials for industrial applications. Amsterdam: Elsevier; 2018.

    Google Scholar 

  27. Zheng W, Luo B, Hu X. The determinants of farmers’ fertilizers and pesticides use behavior in China: an explanation based on label effect. J Clean Prod. 2020;272: 123054.

    Article  Google Scholar 

  28. Kaur R, Mavi GK, Raghav S, Khan I. Pesticides classification and its impact on environment. Int J Curr Microbiol Appl Sci. 2019;8(3):1889–97.

    Article  CAS  Google Scholar 

  29. Kole, R. K. Improved pesticide formulation for sustainable crop protection. In: Ensuring food safety, security and sustainability through crop protection; 2021, vol. 5, p. 50–5. ISBN: 978-81-950908-4-6.

  30. Zheng L, Cao C, Chen Z, Cao L, Huang Q, Song B. Efficient pesticide formulation and regulation mechanism for improving the deposition of droplets on the leaves of rice (Oryza sativa L.). Pest Manag Sci. 2021;77(7):3198–207.

    Article  CAS  PubMed  Google Scholar 

  31. Chen H, Zhi H, Liang J, Yu M, Cui B, Zhao X, Sun C, Wang Y, Cui H, Zeng Z. Development of leaf-adhesive pesticide nanocapsules with pH-responsive release to enhance retention time on crop leaves and improve utilization efficiency. J Mater Chem B. 2021;9(3):783–92.

    Article  CAS  PubMed  Google Scholar 

  32. Pang Y, Qin Z, Wang S, Yi C, Zhou M, Lou H, Qiu X. Preparation and application performance of lignin-polyurea composite microcapsule with controlled release of avermectin. Colloid Polym Sci. 2020;298(8):1001–12.

    Article  CAS  Google Scholar 

  33. Zhu H, Shen Y, Cui J, Wang A, Li N, Wang C, Cui B, Sun C, Zhao X, Wang C, Gao F, Zhan S, Guo L, Zhang L, Zeng Z, Wang Y, Cui H. Avermectin loaded carboxymethyl cellulose nanoparticles with stimuli-responsive and controlled release properties. Ind Crop Prod. 2020;152: 112497.

    Article  CAS  Google Scholar 

  34. Kaziem AE, Gao Y, Zhang Y, Qin X, Xiao Y, Zhang Y, You H, Li J, He S. α-amylase triggered carriers based on cyclodextrin anchored hollow mesoporous silica for enhancing insecticidal activity of avermectin against Plutella xylostella. J Hazard Mater. 2018;359:213–21.

    Article  CAS  PubMed  Google Scholar 

  35. Zhou M, Xiong Z, Yang D, Pang Y, Wang D, Qiu X. Preparation of slow release nanopesticide microspheres from benzoyl lignin. Holzforschung. 2018;72(7):599–607.

    Article  CAS  Google Scholar 

  36. Liu B, Zhang J, Chen C, Wang D, Tian G, Zhang G, Cai D, Wu Z. Infrared-light-responsive controlled-release pesticide using hollow carbon microspheres@ polyethylene glycol/α-cyclodextrin gel. J Agric Food Chem. 2021;69(25):6981–8.

    Article  CAS  PubMed  Google Scholar 

  37. Wu D, Qin M, Xu D, Wang L, Liu C, Ren J, Zhou G, Chen C, Yang F, Li Y, Zhao Y, Huang R, Pourtaheri S, Kang C, Kamata M, Chen ISY, He Z, Wen J, Chen W, Lu Y. A bioinspired platform for effective delivery of protein therapeutics to the central nervous system. Adv Mater. 2019;31(18):1807557.

    Article  CAS  Google Scholar 

  38. Heidary M, Karimzadeh J, Jafari S, Negahban M, Shakarami J. Aphicidal activity of urea–formaldehyde nanocapsules loaded with the Thymus daenensis Celak essential oil on Brevicoryne brassicae L. Int J Trop Insect Sci. 2021.

    Article  Google Scholar 

  39. Zheng F, Li Y, Zhang Z, Jia J, Hu P, Zhang C, Xu H. Novel strategy with an eco-friendly polyurethane system to improve rainfastness of tea saponin for highly efficient rice blast control. J Clean Prod. 2020;264: 121685.

    Article  CAS  Google Scholar 

  40. Luo J, Zhang DX, Jing T, Liu G, Cao H, Li BX, Hou Y, Liu F. Pyraclostrobin loaded lignin-modified nanocapsules: delivery efficiency enhancement in soil improved control efficacy on tomato Fusarium crown and root rot. Chem Eng J. 2020;394: 124854.

    Article  CAS  Google Scholar 

  41. Xiang Y, Zhang G, Chi Y, Cai D, Wu Z. Fabrication of a controllable nanopesticide system with magnetic collectability. Chem Eng J. 2017;328:320–30.

    Article  CAS  Google Scholar 

  42. Shan Y, Cao L, Xu C, Zhao P, Cao C, Li F, Xu B, Huang Q. Sulfonate-functionalized mesoporous silica nanoparticles as carriers for controlled herbicide diquat dibromide release through electrostatic interaction. Int J Mol Sci. 2019;20(6):1330.

    Article  CAS  PubMed Central  Google Scholar 

  43. Xiang Y, Zhang G, Chen C, Liu B, Cai D, Wu Z. Fabrication of a pH-responsively controlled-release pesticide using an attapulgite-based hydrogel. ACS Sustainable Chem Eng. 2018;6(1):1192–201.

    Article  CAS  Google Scholar 

  44. Kabir M, Tisha F, Nayan H, Islam M, Kashem M, Uddin M, Islam M, Meah M. Determining an effective and economic fungicide spray schedule for reducing blast of wheat. Int J Agr Innov Innov Technol. 2021;11(1):10–6.

    Article  Google Scholar 

  45. Li H, Jing T, Li T, Huang X, Gao Y, Zhu J, Lin J, Zhang P, Li B, Mu W. Ecotoxicological effects of pyraclostrobin on tilapia (Oreochromis niloticus) via various exposure routes. Environ Pollut. 2021;285: 117188.

    Article  CAS  PubMed  Google Scholar 

  46. Li M, Xu W, Hu D, Song B. Preparation and application of pyraclostrobin microcapsule formulations. Colloid Surface A. 2018;553:578–85.

    Article  CAS  Google Scholar 

  47. Chi Y, Chen C, Zhang G, Ye Z, Su X, Ren X, Wu Z. Fabrication of magnetic-responsive controlled-release herbicide by a palygorskite-based nanocomposite. Colloids Surf, B. 2021;208: 112115.

    Article  CAS  Google Scholar 

  48. Wu F, Harper BJ, Crandon LE, Harper SL. Assessment of Cu and CuO nanoparticle ecological responses using laboratory small-scale microcosms. Environ Sci Nano. 2020;7(1):105–15.

    Article  CAS  PubMed  Google Scholar 

  49. Jang S, Mergaert P, Ohbayashi T, Ishigami K, Shigenobu S, Itoh H, Kikuchi Y. Dual oxidase enables insect gut symbiosis by mediating respiratory network formation. PNAS. 2021;118(10): e2020922118.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. Lu Z, Deng J, Wang H, Zhao X, Luo Z, Yu C, Zhang Y. Multifunctional role of a fungal pathogen-secreted laccase 2 in evasion of insect immune defense. Environ Microbiol. 2021;23(2):1256–74.

    Article  CAS  PubMed  Google Scholar 

  51. Bharani RSA, Namasivayam SKR. Biogenic silver nanoparticles mediated stress on developmental period and gut physiology of major lepidopteran pest Spodoptera litura (Fab.) (Lepidoptera: Noctuidae)—an eco-friendly approach of insect pest control. J Environ Chem Eng. 2017;5(1):453–67.

    Article  CAS  Google Scholar 

  52. Meng X, Abdlli N, Wang N, Lü P, Nie Z, Dong X, Lu S, Chen K. Effects of Ag nanoparticles on growth and fat body proteins in silkworms (Bombyx mori). Biol Trace Elem Res. 2017;180(2):327–37.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. Anandhi S, Saminathan VR, Yasotha P, Saravanan PT, Rajanbabu V. Nano-pesticides in pest management. J Entomol Zool Stud. 2020;8(4):685–90.

    Google Scholar 

  54. de la Rosa G, Vázquez-Núñez E, Molina-Guerrero C, et al. Interactions of nanomaterials and plants at the cellular level: current knowledge and relevant gaps. Nanotechnol Environ Eng. 2021;6:7.

    Article  CAS  Google Scholar 

  55. Faiz MB, Amal R, Marquis CP, Harry EJ, Sotiriou GA, Rice SA, Gunawan C. Nanosilver and the microbiological activity of the particulate solids versus the leached soluble silver. Nanotoxicology. 2018;12(3):263–73.

    Article  CAS  PubMed  Google Scholar 

  56. Tang S, Zheng J. Antibacterial activity of silver nanoparticles: structural effects. Adv Healthc Mater. 2018;7(13):1701503.

    Article  CAS  Google Scholar 

  57. Shafie RM, Salama AM, Farroh KY. Silver nanoparticles activity against tomato spotted wilt virus. Middle East J Appl Sci. 2018;7:1251–67.

    Google Scholar 

  58. Campos EV, Proença PL, Oliveira JL, Bakshi M, Abhilash PC, Fraceto LF. Use of botanical insecticides for sustainable agriculture: future perspectives. Ecol Indic. 2019;105:483–95.

    Article  CAS  Google Scholar 

  59. Zheng L, Cao C, Cao L, Chen Z, Huang Q, Song B. Bounce behavior and regulation of pesticide solution droplets on rice leaf surfaces. J Agric Food Chem. 2018;66(44):11560–8.

    Article  CAS  PubMed  Google Scholar 

  60. Zhao X, Cui H, Wang Y, Sun C, Cui B, Zeng Z. Development strategies and prospects of nano-based smart pesticide formulation. J Agric Food Chem. 2018;66(26):6504–12.

    Article  CAS  PubMed  Google Scholar 

  61. Liu B, Wang Y, Yang F, Wang X, Shen H, Cui H, Wu D. Construction of a controlled-release delivery system for pesticides using biodegradable PLA-based microcapsules. Colloid Surface B. 2016;144:38–45.

    Article  CAS  Google Scholar 

  62. Wang A, Wang Y, Sun C, Wang C, Cui B, Zhao X, Zeng Z, Yao J, Yang D, Liu G. Fabrication, characterization, and biological activity of avermectin nano-delivery systems with different particle sizes. Nanoscale Res Lett. 2018;13(1):1–7.

    Article  CAS  Google Scholar 

  63. Li W, Wang Q, Zhang F, Shang H, Bai S, Sun J. pH-sensitive thiamethoxam nanoparticles based on bimodal mesoporous silica for improving insecticidal efficiency. Roy Soc open Sci. 2021;8(2): 201967.

    Article  CAS  Google Scholar 

  64. Yin Y, Yang M, Xi J, Cai W, Yi Y, He G, Dai Y, Zhou T, Jiang M. A sodium alginate-based nano-pesticide delivery system for enhanced in vitro photostability and insecticidal efficacy of phloxine B. Carbohydr Polym. 2020;247: 116677.

    Article  CAS  PubMed  Google Scholar 

  65. Wang Y, Song S, Chu X, Feng W, Li J, Huang X, Zhou N, Shen J. A new temperature-responsive controlled-release pesticide formulation–poly (N-isopropylacrylamide) modified graphene oxide as the nanocarrier for lambda-cyhalothrin delivery and their application in pesticide transportation. Colloid Surface A. 2021;612: 125987.

    Article  CAS  Google Scholar 

  66. Chun S, Feng J. Preparation of abamectin nanoparticles by flash nanoprecipitation for extended photostability and sustained pesticide release. ACS Appl Nano Mater. 2021;4(2):1228–34.

    Article  CAS  Google Scholar 

  67. Chen XX, Liu YM, Zhao QY, Cao WQ, Chen XP, Zou CQ. Health risk assessment associated with heavy metal accumulation in wheat after long-term phosphorus fertilizer application. Environ Pollut. 2020;262: 114348.

    Article  CAS  PubMed  Google Scholar 

  68. Zhu Q, Liu X, Hao T, Zeng M, Shen J, Zhang F, de Vries W. Cropland acidification increases risk of yield losses and food insecurity in China. Environ Pollut. 2020;256: 113145.

    Article  CAS  PubMed  Google Scholar 

  69. Lowe BS, Leer DR, Frey JW, Caskey BJ. Occurrence and distribution of algal biomass and its relation to nutrients and selected basin characteristics in Indiana streams, 201–205. Sci Invest Rep. 2008.

    Article  Google Scholar 

  70. Singh MD. Nano-fertilizers is a new way to increase nutrients use efficiency in crop production. Int J Agri Sci 2017; 9(7), 3831–83.

  71. Djaya L, Istifadah N, Hartati S, Joni IM. In vitro study of plant growth promoting rhizobacteria (PGPR) and endophytic bacteria antagonistic to Ralstonia solanacearum formulated with graphite and silica nano particles as a biocontrol delivery system (BDS). Biocatal Agric Biotechnol. 2019;19: 101153.

    Article  Google Scholar 

  72. Benzon HRL, Rubenecia MRU, Ultra VU Jr, Lee SC. Nano-fertilizer affects the growth, development, and chemical properties of rice. Int J Agro and Agri Res. 2015;7(1):105–17.

    Google Scholar 

  73. Haydar MS, Ghosh S, Mandal P. Application of iron oxide nanoparticles as micronutrient fertilizer in mulberry propagation. J Plant Growth Regul. 2021.

    Article  Google Scholar 

  74. Raliya R, Saharan V, Dimkpa C, Biswas P. Nanofertilizer for precision and sustainable agriculture: current state and future perspectives. J Agric Food Chem. 2017;66(26):6487–503.

    Article  CAS  PubMed  Google Scholar 

  75. Li D, Zhou C, Zou N, Wu Y, Zhang J, An Q, Li J, Pan C. Nanoselenium foliar application enhances biosynthesis of tea leaves in metabolic cycles and associated responsive pathways. Environ Pollut. 2021;273: 116503.

    Article  CAS  PubMed  Google Scholar 

  76. Alimohammadi M, Panahpour E, Naseri A. Assessing the effects of urea and nano-nitrogen chelate fertilizers on sugarcane yield and dynamic of nitrate in soil. Soil Sci Plant Nutr. 2020;66(2):352–9.

    Article  CAS  Google Scholar 

  77. Rajonee AA, Zaman S, Huq SMI. Preparation, characterization and evaluation of efficacy of phosphorus and potassium incorporated nano fertilizer. Adv Nanopart. 2017;6(02):62.

    Article  CAS  Google Scholar 

  78. Chen D, Szostak P, Wei Z, Xiao R. Reduction of orthophosphates loss in agricultural soil by nano calcium sulfate. Sci Total Environ. 2016;539:381–7.

    Article  CAS  PubMed  Google Scholar 

  79. Ahanger MA, Qi M, Huang Z, Xu X, Begum N, Qin C, Zhang C, Ahmad N, Mustafa N, Ashraf M, Zhang L. Improving growth and photosynthetic performance of drought stressed tomato by application of nano-organic fertilizer involves up-regulation of nitrogen, antioxidant and osmolyte metabolism. Ecotoxicol Environ Saf. 2021;216: 112195.

    Article  CAS  PubMed  Google Scholar 

  80. Kalia A, Kaur H. Nano-biofertilizers: Harnessing dual benefits of nano-nutrient and bio-fertilizers for enhanced nutrient use efficiency and sustainable productivity. In: Pudake R, Chauhan N, Kole C, editors. Nanoscience for sustainable agriculture. Cham: Springer; 2019.

    Chapter  Google Scholar 

  81. Yaseen R, Ahmed AIS, Omer AM, Agha MKM, Emam TM. Nano-fertilizers: Bio-fabrication, application and biosafety. Nov Res Microbiol J. 2020; 4(4), 884–900.

  82. Tang FH, Lenzen M, McBratney A, Maggi F. Risk of pesticide pollution at the global scale. Nat Geosci. 2021;14(4):206–10.

    Article  CAS  Google Scholar 

  83. Tackenberg MC, Giannoni-Guzmán MA, Sanchez-Perez E, Doll CA, Agosto-Rivera JL, Broadie K, Moore D, McMahon DG. Neonicotinoids disrupt circadian rhythms and sleep in honey bees. Sci Rep. 2020;10(1):1–10.

    Article  CAS  Google Scholar 

  84. Dewen Q. Research progress and prospect of bio-pesticides. Plant Protect. 2013;39(5):81–9.

    Article  CAS  Google Scholar 

  85. Devi PV, Duraimurugan P, Chandrika K. Chapter 10-Bacillus thuringiensis-based nanopesticides for crop protection. Nano-biopesticides today and future perspectives, Academic Press. 2019, p. 249–60.

  86. Zaki AM, Zaki AH, Farghali AA, Abdel-Rahim EF. Sodium titanate-bacillus as a new nanopesticide for cotton leaf-worm. J Pure Appl Microbiol. 2017;11(2):725–32.

    Article  CAS  Google Scholar 

  87. de Oliveira JL, Fraceto LF, Bravo A, Polanczyk RA. Encapsulation strategies for Bacillus thuringiensis: from now to the future. J Agric Food Chem. 2021;69(16):4564–77.

    Article  CAS  PubMed  Google Scholar 

  88. Hersanti, Djaya L, Hidayat Y, Pratama LS, Joni IM. The effectiveness of suspension of Beauveria bassiana mixed with silica nanoparticles (NPs.) and carbon fiber in controlling Spodoptera litura. In AIP Conference Proceedings (Vol. 2219, No. 1, p. 080011); 2020. AIP Publishing LLC.

  89. Gahukar RT, Das RK. Plant-derived nanopesticides for agricultural pest control: challenges and prospects. Nanotechnol Environ Eng. 2020;5(1):1–9.

    Article  CAS  Google Scholar 

  90. Cinteza LO, Scomoroscenco C, Voicu SN, Nistor CL, Nitu SG, Trica B, Jecu M, Petcu C. Chitosan-stabilized Ag nanoparticles with superior biocompatibility and their synergistic antibacterial effect in mixtures with essential oils. Nanomaterials. 2018;8(10):826.

    Article  CAS  PubMed Central  Google Scholar 

  91. Cui J, Sun C, Wang A, Wang Y, Zhu H, Shen Y, Li N, Zhao X, Cui B, Wang C, Gao F, Zeng Z, Cui H. Dual-functionalized pesticide nanocapsule delivery system with improved spreading behavior and enhanced bioactivity. Nanomaterials. 2020;10(2):220.

    Article  CAS  PubMed Central  Google Scholar 

  92. Dagar A, Weksler A, Friedman H, Lurie S. Gibberellic acid (GA3) application at the end of pit ripening: effect on ripening and storage of two harvests of ‘September Snow’peach. Sci Hortic. 2012;140:125–30.

    Article  CAS  Google Scholar 

  93. Hafez IH, Osman AR, Sewedan EA, Berber MR. Tailoring of a potential nanoformulated form of gibberellic acid: synthesis, characterization, and field applications on vegetation and flowering. J Agric Food Chem. 2018;66(31):8237–45.

    Article  CAS  PubMed  Google Scholar 

  94. Katiyar D, Hemantaranjan A, Singh B, Bhanu AN. A future perspective in crop protection: chitosan and its oligosaccharides. Adv Plants Agric Res. 2014;1(1):00006.

    Article  Google Scholar 

  95. Asgari-Targhi G, Iranbakhsh A, Ardebili ZO, Tooski AH. Synthesis and characterization of chitosan encapsulated zinc oxide (ZnO) nanocomposite and its biological assessment in pepper (Capsicum annuum) as an elicitor for in vitro tissue culture applications. Int J Biol Macromol. 2021;189:170–82.

    Article  CAS  PubMed  Google Scholar 

  96. Ji Y, Ma S, Lv S, Wang Y, Lü S, Liu M. Nanomaterials for targeted delivery of agrochemicals by an all-in-one combination strategy and deep learning. ACS Appl Mater Interfaces. 2021;13(36):43374–86.

    Article  CAS  PubMed  Google Scholar 

  97. Zhang J, Khan SA, Heckel DG, Bock R. Next-generation insect-resistant plants: RNAi-mediated crop protection. Trends Biotechnol. 2017;35(9):871–82.

    Article  CAS  PubMed  Google Scholar 

  98. Nunes CC, Dean RA. Host-induced gene silencing: a tool for understanding fungal host interaction and for developing novel disease control strategies. Mol Plant Pathol. 2012;13(5):519–29.

    Article  CAS  PubMed  Google Scholar 

  99. Fukudome A, Fukuhara T. Plant dicer-like proteins: double-stranded RNA-cleaving enzymes for small RNA biogenesis. J Plant Res. 2017;130(1):33–44.

    Article  CAS  PubMed  Google Scholar 

  100. Kim VN. Small RNAs: classification, biogenesis, and function. Mol Cells. 2005;19(1):1–15.

    Article  CAS  PubMed  Google Scholar 

  101. Haussecker D, Huang Y, Lau A, Parameswaran P, Fire AZ, Kay MA. Human tRNA-derived small RNAs in the global regulation of RNA silencing. RNA. 2010;16(4):673–95.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  102. Xie Z, Johansen LK, Gustafson AM, Kasschau KD, Lellis AD, Zilberman D, Jacobsen SE, Carrington JC. Genetic and functional diversification of small RNA pathways in plants. PLoS Biol. 2004;2(5): e104.

    Article  PubMed  PubMed Central  Google Scholar 

  103. Yan S, Ren BY, Shen J. Nanoparticle-mediated double-stranded RNA delivery system: A promising approach for sustainable pest management. Insect Sci. 2021;28(1):21–34.

    Article  CAS  PubMed  Google Scholar 

  104. Mitter N, Worrall EA, Robinson KE, Li P, Jain RG, Taochy C, Fletcher SJ, Carroll BJ, Lu GM, Xu ZP. Clay nanosheets for topical delivery of RNAi for sustained protection against plant viruses. Nat Plants. 2017;3(2):1–10.

    Article  CAS  Google Scholar 

  105. Kwak S, Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev K, Snell KD, Seo JS, Chua N, Strano MS. Chloroplast-selective gene delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers. Nat Nanotechnol. 2019;14(5):447–55.

    Article  CAS  PubMed  Google Scholar 

  106. Demirer GS, Zhang H, Matos JL, Goh NS, Cunningham FJ, Sung Y, Chang R, Aditham AJ, Chio L, Cho M, Staskawicz B, Landry MP. High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants. Nat Nanotechnol. 2019;14(5):456–64.

    Article  CAS  PubMed  Google Scholar 

  107. Gaspar T, Kevers C, Penel C, Greppin H, Reid DM, Thorpe TA. Plant hormones and plant growth regulators in plant tissue culture. In vitro Cell Dev-Plant. 1996;32(4):272–89.

    Article  CAS  Google Scholar 

  108. Chen J, Cao S, Xi C, Chen Y, Li X, Zhang L, Wang G, Chen Y, Chen Z. A novel magnetic β-cyclodextrin modified graphene oxide adsorbent with high recognition capability for 5 plant growth regulators. Food Chem. 2018;239:911–9.

    Article  CAS  PubMed  Google Scholar 

  109. Li N, Wu D, Li X, Zhou X, Fan G, Li G, Wu Y. Effective enrichment and detection of plant growth regulators in fruits and vegetables using a novel magnetic covalent organic framework material as the adsorbents. Food Chem. 2020;306: 125455.

    Article  CAS  PubMed  Google Scholar 

  110. Santo Pereira AE, Silva PM, Oliveira JL, Oliveira HC, Fraceto LF. Chitosan nanoparticles as carrier systems for the plant growth hormone gibberellic acid. Colloid Surface B. 2017;150:141–52.

    Article  CAS  Google Scholar 

  111. Khodakovskaya MV, Kim BS, Kim JN, Alimohammadi M, Dervishi E, Mustafa T, Cernigla CE. Carbon nanotubes as plant growth regulators: effects on tomato growth, reproductive system, and soil microbial community. Small. 2013;9(1):115–23.

    Article  CAS  PubMed  Google Scholar 

  112. Chakravarty D, Erande MB, Late DJ. Graphene quantum dots as enhanced plant growth regulators: effects on coriander and garlic plants. J Sci Food Agr. 2015;95(13):2772–8.

    Article  CAS  Google Scholar 

  113. Gregg PC, Del Socorro AP, Landolt PJ. Advances in attract-and-kill for agricultural pests: beyond pheromones. Annu Rev Entomol. 2018;63:453–70.

    Article  CAS  PubMed  Google Scholar 

  114. Larson NR, Strickland J, Shields VD, Zhang A. Controlled-release dispenser and dry trap developments for Drosophila suzukii detection. Front Ecol Evol. 2020;8:45.

    Article  Google Scholar 

  115. Seo SM, Lee JM, Lee HY, An J, Choi SJ, Lim WT. Synthesis of nanoporous materials to dispense pheromone for trapping agricultural pests. J Porous Mat. 2016;23(2):557–62.

    Article  CAS  Google Scholar 

  116. Correia PRC, Santana JS, Ramos IG, Sant Ana AEG, Goulart HF, Druzian JI. Development of membranes composed of poly (butylene adipate-co-terephthalate) and activated charcoal for use in a controlled release system of pheromone. J Polym Environ. 2019;27(8):1781–9.

    Article  CAS  Google Scholar 

  117. Bhagat D, Samanta SK, Bhattacharya S. Efficient management of fruit pests by pheromone nanogels. Sci Rep. 2013;3(1):1–8.

    Article  CAS  Google Scholar 

  118. Rai M, Ribeiro C, Mattoso L, Duran N. Nanotechnologies in food and agriculture, Vol. 33, Cham/Heidelberg/New York/Dordrecht/London: Springer; 2015.

  119. Juárez-Maldonado A, Tortella G, Rubilar O, Fincheira P, Benavides-Mendoza A. Biostimulation and toxicity: the magnitude of the impact of nanomaterials in microorganisms and plants. J Adv Res. 2021;31:113–26.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  120. Yang X, He Q, Guo F, Sun X, Zhang J, Chen Y. Impacts of carbon-based nanomaterials on nutrient removal in constructed wetlands: Microbial community structure, enzyme activities, and metabolism process. J Hazard Mater. 2021;401: 123270.

    Article  CAS  PubMed  Google Scholar 

  121. Zhang P, Guo Z, Ullah S, Melagraki G, Afantitis A, Lynch I. Nanotechnology and artificial intelligence to enable sustainable and precision agriculture. Nat Plants. 2021;7(7):864–76.

    Article  PubMed  Google Scholar 

  122. Kah M, Kookana RS, Gogos A, Bucheli TD. A critical evaluation of nanopesticides and nanofertilizers against their conventional analogues. Nature Nanotech. 2018;13(8):677–84.

    Article  CAS  Google Scholar 

  123. StatNano. Home | Nanotechnology Products Database; 2021. | Accessed 25 Nov 2021.

  124. Younis SA, Kim KH, Shaheen SM, Antoniadis V, Tsang YF, Rinklebe J, Deep A, Brown RJ. Advancements of nanotechnologies in crop promotion and soil fertility: benefits, life cycle assessment, and legislation policies. Renew Sust Energ Rev. 2021;152: 111686.

    Article  CAS  Google Scholar 

  125. Kah M, Tufenkji N, White JC. Nano-enabled strategies to enhance crop nutrition and protection. Nat Nanotechnol. 2019;14(6):532–40.

    Article  CAS  PubMed  Google Scholar 

  126. Beumer K. On the elusive nature of the public. Nat Nanotechnol. 2019;14(6):510–2.

    Article  CAS  PubMed  Google Scholar 

Download references


We are grateful for financial support from the National Key R&D Program of China (2021YFA0716704), the National Natural Science Foundation of China (31701825), the National Key R&D Program of China (2016YFD0200500), and the Agricultural Science and Technology Innovation Program (CAAS-ZDRW202008), the Basic Scientific Research Foundation of National non-Profit Scientific Institute of China (BSRF201907), the National Key R&D Program of China (2017YFD0200300, 2017YFD0201207, 2018YFD0200200), and Technology Program for Water Pollution Control and Treatment (No. 2017ZX07101003).

Author information

Authors and Affiliations



CA and CS contributed equally to this work. The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Haixin Cui or Yan Wang.

Ethics declarations

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

An, C., Sun, C., Li, N. et al. Nanomaterials and nanotechnology for the delivery of agrochemicals: strategies towards sustainable agriculture. J Nanobiotechnol 20, 11 (2022).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI:


  • Nanomaterials
  • Agrochemicals
  • Nanotechnology
  • Nanoformulations
  • Sustainable agriculture