Fig. 3

A The 4T1 cells were cultured for 2 h and 6 h with DIMP was observed with laser confocal microscopy. B The average fluorescence intensity of DOX and ICG was quantified (Scale bars: 20 μm). C 4T1 cells were incubated with DIMP for 6 h and stained with JC-1. Red fluorescence indicated JC-1 aggregates, green fluorescence indicated JC-1 monomers (Scale bar: 50 μm). D Average fluorescence intensity of JC-1 monomer in each group of cells. E Schematic diagram of DNA damage experiment. F 4T1 cells were incubated with DOX, ICG and DIMP, and then DNA damage was observed with or without laser treatment. γ-H2AX (green) and DAPI (blue) were used for staining and marking (Scale bar: 20 μm). G The average fluorescence intensity of γ-H2AX in each group. H Signal intensity distribution of DOX, DIMP, ICG + L, DIMP + L channels