Fig. 2
Attenuation of Aβ1–42 aggregation by PLGA at different temperatures. PLGA dose-dependently (5–25 µM) attenuates spontaneous aggregation of 10 µM Aβ1–42 as revealed by ThT kinetic assays and respective fluorescence images/quantification over 24 h incubation at 27 °C (A–D), 37 °C (E–H) and 40 °C (I–L). The corrosponding rate constant of the kinetic reaction “K” is shown as inset in each plot. ThT stained fluorescence images (B, C, F, G, J, K) and the corresponding quantification (D, H, L) of PLGA untreated and treated Aβ1–42 samples were taken after 24 h incubation at 27 °C, 37 °C and 40 °C. Histograms representing the attenuated fluorescence values at lag-time (M) and at saturation (N) of Aβ1–42 kinetic reaction in the absence and presence of 5-25 µM PLGA at different temperatures