Fig. 6

Analysis of tumor sections shows distribution of mIgG1-IH18 radiographic and fluorescent signal and PD-L1 expression. A Physically adjacent tissue sections of a tumor from a mIgG1-IH18 treated mouse are stained with H&E, PD-L1 immunostaining, or where fluorescent and radiographic signal is acquired. PD-L1, autoradiography and fluorescence patterns are overlapping, demonstrating co-localization of these signals. B Fluorescence microscopy shows localization of mIgG1-IH18 with cellular resolution (DAPI in blue, Cy5 fluorescence in purple and PD-L1 immunofluorescent staining in green in the merge image)