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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Extracellular microparticles derived from hepatic progenitor cells deliver a death signal to hepatoma-initiating cells

Fig. 5

HPC-derived MPs without death signals have no effect on hepatocarcinogenesis. A Experimental outline for producing MPs without apoptotic signals (DOXO-MPs and RT-MPs). WB-F344 cells were treated with 100 µg/ml doxorubicin or 20 Gy radiation. DOXO-MPs and RT-MPs were isolated before the cells underwent apoptosis. BF Sprague Dawley rats, which were treated with DEN for 6 weeks, were intrasplenically injected with 40 µg of DOXO-MPs and RT-MPs. Injections were administered twice every week for 7 weeks. DEN treatment was also continued during this time. After 13 weeks, the rats were sacrificed to observe the development of hepatocellular carcinoma (HCC). Representative images of rat livers are shown. Typical tumor nodes are marked by the asterisks (B). The maximum tumor volume per liver in the indicated groups (C). The number of HCC nodules per liver (D). The liver-to-body weight ratio (E). The tumor incidence in the different treatment groups (F). G Images of H&E-stained liver sections showing the histological structure and inflammatory response after the indicated treatments. Black asterisks indicate accumulation of inflammatory cells

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