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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Functionalizing silica sol–gel with entrapped plant virus-based immunosorbent nanoparticles

Fig. 2

Purification and immobilization of fluorescently labelled plant virions a SDS-PAGE results of an ultracentrifuge-based purification of wt-TMV from Nicotiana benthamiana leaf tissue, which is then chemically conjugated with Cy5 dye in a two-step reaction to generate Cy5-TMV. Lane definitions: I—filtered plant extract, II—PEG-precipitated pellet resuspension, III—microfuge pellet, IV—ultracentrifuge pellet resuspension, V—ultracentrifuge wash, VI—initial wt-TMV pre-conjugation, VII—Cy5-TMV post-conjugation. b UV–Vis absorbance spectra of wt-TMV and Cy5-TMV. c Negative stain transmission electron microscope image of the Cy5-TMV. d Normalized areal fluorescence per 2 µL volume silica bead containing Cy5-TMV over 48 h. Beads were exchanged into fresh PBS buffer after each measurement. Error bars represent one standard deviation with biological triplicate. e Fluorescent microscope images of the silica sol–gel beads containing Cy5-TMV over 48 h of submerged gel wash. Scale bars represent 500 µm

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