Fig. 6
Effects of Ti3C2 nanosheets on trophoblast proliferation and function of syncytiotrophoblasts. a Histological sections and H&E staining (scale bar = 200 μm) of placenta tissue collected on pregnant day 12. LAB, labyrinth; SPT, spongiotrophoblast; MD, maternal decidua. b The organ coefficient of placenta on pregnant day 12 at different doses (n = 9 per group). c The placental efficiency on GD12 at different doses (n = 9 per group). d Quantification of the LAB area, SPT area, and MD area of three placentae per dose. e Histological sections and immunohistochemistry staining for VEGFR2 and Ki67 (scale bars = 500 μm and 50 μm) on GD12 at each dose. f The levels of serum β-CG on GD10 and GD12 at different doses (n = 3 mice per group). g The levels of serum PIGF on GD10 and GD12 at different doses (n = 3 mice per group). h The levels of serum sFlt-1 on GD10 and GD12 at different doses (n = 3 mice per group). Data were analysed by two-way ANOVA. Data in b–h are shown as means ± SD. **p < 0.01, ***p < 0.001, ****p < 0.000 vs. Control group