Fig. 4
IL-10 activates osteoblast differentiation and inhibited osteoclast differentiation. a Osteogenesis-related genes were upregulated in BMSCs treated with IL-10 recombinant proteins quantified by RT-PCR analysis. b ALP staining in BMSCs following treated by IL-10 recombinant proteins for 14 d. c ALP activity was examined via an ALP assay kit. d Alizarin red-mediated calcium staining in BMSCs following treated by IL-10 recombinant proteins for 21 d. e Alizarin red staining was extracted by cetylpyridinium chloride and quantified by spectrophotometer. f Osteoclastogenesis-related genes were inhibited in BMDM induced osteoclasts treated with IL-10 by RT-PCR analysis. g TRAP staining was performed in osteoclasts induced by BMDM treated with IL-10 for 7 d. The number (h) and area (i) of TRAP-positive cells was calculated. Data are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001