Fig. 5

M2-Exos elevate IL-10 cytokines expression of BMDM and BMSCs via delivering exosomal IL-10 mRNA to the cells directly. a Total RNA was extracted from exosomes and quantified by RT-PCR analysis. Surface IL-10R expression of BMSCs (b) and BMDM (c) was monitored by flow cytometry. d The expression of IL-10 was measured in BMSCs or BMDM treated by PBS, 50 μg/ml M2-Exos, or 100 μg/ml M2-Exos by RT-PCR analysis. e IL-10 cytokines in culture supernatant of BMSCs or BMDM were measured by ELISA analysis