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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Exosomes derived from reparative M2-like macrophages prevent bone loss in murine periodontitis models via IL-10 mRNA

Fig. 6

M2-Exos promote osteogenesis and suppress osteoclastogenesis via IL-10/IL-10R pathway. a Osteogenesis-related genes were quantified in BMSCs treated by PBS, M2-Exos, or M2-Exos with anti-IL-10R antibody by RT-PCR analysis. b ALP staining in BMSCs following treated by PBS, M2-Exos, or M2-Exos with anti-IL-10R antibody for 14 d. c ALP activity was examined via an ALP assay kit. d Alizarin red-mediated calcium staining in BMSCs following treated by PBS, M2-Exos, or M2-Exos with anti-IL-10R antibody for 21 d. e Alizarin red staining was extracted by cetylpyridinium chloride and quantified by spectrophotometer. f Osteoclastogenesis-related genes were measured in BMDM induced osteoclasts treated by PBS, M2-Exos, or M2-Exos with anti-IL-10R antibody by RT-PCR analysis. g TRAP staining was performed in osteoclasts induced by BMDM treated by PBS, M2-Exos, or M2-Exos with anti-IL-10R antibody for 7 d. The number (h) and area (i) of TRAP-positive cells was calculated. Data are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001

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