Fig. 3

H₂O₂ scavenging and gene transfection of NPs in HUVECs. A Schematic illustration of NP’s ROS scavenging and pVEGF/pNGF co-transfection in cells. B Cellular uptake of naked-Coumarin and Coumarin-NPs for 0 h, 6 h, 12 h and 24 h. C Lysosomal escape of Coumarin-NPs. D H₂O₂ scavenging of HUVECs after incubating Blank-NPs 24 h. E Cell viability of HUVECs after incubation with different concentrations of Blank-NPs in 10 µM H₂O₂. The data are presented as mean ± SD (n = 5, ^**P < 0.01, ^***P < 0.001). F Gene co-transfection through NPs