Fig. 5
Enhanced interaction between SH-SY5Ys and HUVECs. A Schematic illustration of interaction between SH-SY5Ys and HUVECs. After SH-SY5Ys was incubated with serum-free medium (Control), supernatant (Sup), supernatant + VEGF/NGF-NPs (Sup + NPs) for 24 h, HUVECs were incubated with supernatant for 24 h. B Cellular uptake of naked-Coumarin and Coumarin-NPs in SH-SY5Ys for 0 h, 6 h, 12 h, 24 h. C Lysosomal escape of Coumarin-NPs in SH-SY5Ys. D H2O2 scavenging of SH-SY5Ys after incubating Blank-NPs in 10 µM LPS. E Migration images of HUVECs after incubating with serum-free medium, Sup and Sup + NPs for 12 h and 24 h. Quantification of cell migration for 12 h (F) and 24 h (G) were also quantified and the data are presented as mean ± SD (n = 4, **P < 0.01, ***P < 0.001). Cell viabilities of HUVECs after incubating with serum-free medium, Sup and Sup + NPs in PBS (H) and 10 µM H2O2 (I) and the data are presented as mean ± SD (n = 5, **P < 0.01, ***P < 0.001)