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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Visualization of the process of a nanocarrier-mediated gene delivery: stabilization, endocytosis and endosomal escape of genes for intracellular spreading

Fig. 3

Improved endocytosis and exocytosis of dsRNA/SPc complex. (a-b) Cellular uptake of naked dseGFP (a) and dseGFP/SPc complex (b). The cells were incubated with dseGFP and dseGFP/SPc complex for 6 h, respectively (dseGFP: 500 ng). Blue: DAPI. Green: dseGFP. (c–e’) Vesicle release of SPc-delivered dseGFP into cytoplasm. The cells were incubated with dseGFP and dseGFP/SPc complex, respectively (dseGFP: 500 ng) for 6 h. A surface plot test was conducted using ImageJ. Three typical regions were marked. f, g’ Endosomal escape of SPc-delivered dsRNA. The cells incubated with SPc-delivered dsRNA for 6 h were imaged. Red: Rab7 marking the late endosome. h–h’ Potential exocytosis of dsRNA/SPc complex. The cells incubated with SPc-delivered dsRNA for 6 h were re-suspended in fresh medium, and then imaged. i Schematic illustration of endocytosis and exocytosis of SPc-delivered dsRNA

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