Fig. 4

Comparative transcriptome analysis of Sf9 cells treated with naked dseGFP and dseGFP/SPc complex. Analysis of differentially expressed genes (DEGs) with volcano plot (a) and GO annotation (b). Cells were collected at 6 h after incubation with dseGFP and dseGFP/SPc complex (dseGFP: 500 ng) for RNA extraction. c Heatmap of the endocytic pathway. d QRT-PCR analysis of the genes related to clathrin-mediated endocytosis. The expression levels of AP2S1, Arf and Chc were determined at various time points after the treatment of dseGFP or dseGFP/SPc complex (dseGFP: 500 ng). Each treatment consisted of 3 replications. The actin and ribosomal protein S15 (RPS-15) were used as reference genes. Asterisk indicates significant difference in gene expression between dseGFP/SPc complex and naked dseGFP treatment according to independent t-test (ns.: no significant difference, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001)