Fig. 6

Luminescence imaging of pulmonary micrometastasis in mice with intravenously inoculated 4T1-GFP tumor cells by LAD NPs. A In vivo luminescence images showing pulmonary metastasis in mice. At various time points after i.v. inoculation of 4T1-GFP cells in mice, 3 mg LAD NPs was administered in each mouse by i.v. injection, immediately followed by luminescence imaging. B Quantitative analysis of pulmonary luminescence intensities in mice at various time points. C Percentages of neutrophils in bronchoalveolar lavage fluid (BALF) of mice after i.v. inoculation of 4T1-GFP cells for different time periods. D Quantified MPO levels in the lungs. E–G Correlation analyses of the luminescence intensity and the number of neutrophils in BALF (E), the MPO level in the lungs (F), or the 4T1-GFP cell counts in the lungs (G). Data are expressed as means ± SD (B, n = 3; C, n = 5; D, n = 6)