Fig. 8
Luminescence imaging of early lung micrometastasis using LAD-PGP NPs in mice with inoculated 4T1-GFP cells. A, B Luminescence images (A) and quantitative analysis of luminescence intensities (A) of mice at different time periods after inoculation of 4T1-GFP cells. Images were acquired at 5 min after i.v. injection of 3 mg LAD-PGP NPs in each mouse. C, D Ex vivo luminescence images (C) and quantified intensities (D) for lung tissues isolated at different time points. E, F Ex vivo images (E) and quantitative analysis (F) of fluorescence intensities in isolated lung tissues. G Analysis of the correlation between GFP fluorescence and LAD-PGP NPs luminescence intensities in lung tissues. H–J Quantified neutrophil counts (H), MPO levels (I), and H2O2 concentrations (J) in BALF from mice at different time points after inoculation with 4T1-GFP cells. K–M Correlations between in vivo luminescence intensities of LAD-PGP NPs and neutrophil counts (K), MPO levels (L), or tumor cell counts (M). Data are expressed as mean ± SD (B, D, F, n = 3; H–J, n = 4)