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Fig.1 | Journal of Nanobiotechnology

Fig.1

From: Engineering stem cells to produce exosomes with enhanced bone regeneration effects: an alternative strategy for gene therapy

Fig.1

HMSCs were genetically engineered by upregulating BMP2 gene expression. a A schematic illustration of genetically engineering hMSCs by liposome mediated BMP2 gene delivery. b, c Fluorescent images of hMSCs transfected by liposome/pGFP-BMP2 plasmid, and transfection efficiency was analyzed by flow cytometry, Scale bar: 100 μm. d mRNA expressions of osteogenic differentiation-related markers (BMP2, Runx2, OSX, ALP, BSP, OPN) in hMSCs were analyzed by qRT-PCR after transfection. Untransfected hMSCs were detected as control, n = 3. *p < 0.05, **p < 0.01

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Journal of Nanobiotechnology

ISSN: 1477-3155

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