Fig. 1

Aging is associated with defects in wound healing and decreasing FMT. A Representative images of wound closure in aged and young groups. B Relative wound area was calculated. C Masson’s trichome staining of wound sections in aged and young groups (scale bar: 200 μm). D % of collagen deposition was calculated. E MMP3, MMP9 and MMP12 mRNA levels were analyzed using qRT-PCR. F Immunofluorescence staining for α-SMA was used to examine the density of myofibroblasts (scale bar: 50 μm). G, H α-SMA and Col1 mRNA levels were analyzed. I Representative results of western blot analysis for α-SMA and Col1. J, K Analysis of western blot results for α-SMA and Col1. ^*P < 0.05 versus Aged by Student’s t-tests (n = 6)