Skip to main content
Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Young fibroblast-derived exosomal microRNA-125b transfers beneficial effects on aged cutaneous wound healing

Fig. 2

ExosomeYoung -guided phenotypic switch to myofibroblasts accelerated wound healing. A Schematic diagram of mouse treatment. Exosomal collection was confirmed using TEM, NTA and western blot analysis. B Representative TEM image. C Size range of exosomes validated using NTA. D Representative western blot images showing that the exosomal markers ALIX, CD9 and TSG101 were expressed in exosomeOld and exosomeYoung. E Representative images of wound closure after local injection of saline (Control), exosomeOld and exosomeYoung. F Relative wound area was calculated. G, H Masson’s trichome staining of wound sections in control, exosomeOld and exosomeYoung groups (scale bar: 200 μm). I Immunofluorescence staining for α-SMA was used to examine the density of myofibroblasts (scale bar: 50 μm). J, K α-SMA and Col1 mRNA levels were analyzed. *P < 0.05 versus exosomeYoung in one-way analysis of variance (n = 6)

Back to article page