Fig. 4
Identification of major mechanisms associated with exosome Young -guided wound healing and FMT. A MiR-125b expression was validated by qRT-PCR in fibroblasts after transfection of miR-125b inhibitor, or miR inhibitor negative control (NC). *P < 0.05 versus miR-125b inhibitor in repeated measures analysis of variance (n = 6). B MiR-125b expression was validated by qRT-PCR in exosomes after inhibiting miR-125b in fibroblasts. *P < 0.05 versus exosomemiR−125b inhibitor in repeated measures analysis of variance (n = 6). C Representative images of wound closure after local injection of saline (Control), exosomeYoung, exosomeYoung+miR−125b inhibitor or exosomeYoung+miR−NC inhibitor. D Relative wound area was calculated. E, F Masson’s trichome staining of wound sections in control, exosomeYoung, exosomesYoung+miR−125b inhibitor or exosomeYoung+miR−NC inhibitor groups (scale bar: 200 μm). G Immunofluorescence staining for α-SMA was used to examine the density of myofibroblasts (scale bar: 50 μm). (H and I) α-SMA and Col1 mRNA levels were analyzed. J, K Western blot analysis of Sirt7 and β-actin protein levels. *P < 0.05 versus exosomeYoung; #P < 0.05 versus exosomeYoung+miR−NC inhibitor in one-way analysis of variance (n = 6)