Fig. 5

Exosome^Young activated fibroblast^Old migration and induced fibrosis. A Schematic illustration of fibroblast^Old treatment. B Cellular proliferation was evaluated by Brdu staining. C, D Cell cycle distribution was analyzed. E Images of migrated fibroblast^Old using Transwell migration assays. F Data are presented as the number of migrated cells. G, I Expression of α-SMA was determined using qRT-PCR and western blot analysis. J Representative images of immunofluorescence staining of α-SMA in fibroblast^Old. K Quantification of α-SMA-positive cells. ^*P < 0.05 versus exosome^Young in repeated measures analysis of variance (n = 6)